HPLCによるシプロフィブル酸エナンチオマーの分析、CYCLOBOND I 2000

column

CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)

mobile phase

[A] acetonitrile; [B] methanol; [C] acetic acid; [D] triethylamine; (90:10:0.3:0.2, A:B:C:D)

flow rate

1 mL/min

column temp.

ambient

detector

UV, 230 nm

injection

1 μL

sample

5 mg/mL in methanol