コンテンツへスキップ
Merck
  • Development of a method for the analysis of seven banned azo-dyes in chilli and hot chilli food samples by pressurised liquid extraction and liquid chromatography with electrospray ionization-tandem mass spectrometry.

Development of a method for the analysis of seven banned azo-dyes in chilli and hot chilli food samples by pressurised liquid extraction and liquid chromatography with electrospray ionization-tandem mass spectrometry.

Talanta (2009-01-29)
Olga Pardo, Vicent Yusà, Nuria León, Agustín Pastor
要旨

An automated, confirmatory and sensitive procedure has been developed and validated for the determination of Sudan (I-IV), Sudan Orange G, Sudan Red 7B and Para Red in hot chilli food samples. The proposed method includes pressurised liquid extraction (PLE) with acetone, gel permeation chromatography (GPC) clean-up and detection by liquid chromatography (LC) coupled to electrospray ionization in positive mode tandem mass spectrometry (ESI-MS-MS). The main parameters affecting the performance of the different ionization sources and PLE parameters were previously optimised using statistical design of experiments (DOE). The method was in-house validated on chilli powder and chilli meat. Linear calibrations were obtained with correlation coefficients R(2)>0.999. The limits of detection (LOD) and quantification (LOQ) of the method were in the ranges of 0.002-0.012 ng g(-1) and 0.006-0.036 ng g(-1), respectively for chilli powder. The decision limit and detection capability were between 0.005-0.022 ng g(-1) and 0.007-0.026 ng g(-1), respectively for chilli meat. Recoveries ranged from 94% to 105%. The applicability of the method to the determination of azo-dyes in hot chilli products was demonstrated.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
オレンジG, for NA electrophoresis
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
オレンジG, certified by the Biological Stain Commission
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
オレンジG, certified by the Biological Stain Commission, Dye content 80 %
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
スダンレッド7B
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
スダンII, Dye content 90 %
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
スダンレッド7B, for microscopy (Bot., Hist.)
カタログ番号
容量
在庫状況
単価
数量
Supelco
スダンII, analytical standard
カタログ番号
容量
在庫状況
単価
数量
Supelco
スダンレッド7B, analytical standard
カタログ番号
容量
在庫状況
単価
数量
Supelco
Sudan IV-d6, analytical standard
カタログ番号
容量
在庫状況
単価
数量