G005739

HPLC Analysis of Silymarin on Ascentis^® Express C18

application for HPLC

特性

• テクニック: HPLC: suitable
• 試験パラメータ: column: Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U); mobile phase: [A] water with 0.1% formic acid; [B] methanol; gradient: held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min; flow rate: 0.6 mL/min; pressure: 4105 psi (283 bar); column temp.: 35 °C; detector: UV, 254 nm; injection: 20 μL; sample: 100 μg/mL in 90:10, water:methanol
• 適合性: application for HPLC
• アプリケーション: vitamins, nutraceuticals, and natural products

詳細

アナリシスノート

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of silymarin.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

アナライト

Taxifolin
Silychristin
Apigenin 7-glucoside
Silydianin
Silybin B
Isosilybin A
Isosilybin B