V4139

抗ビンキュリン ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: Vinculin Antibody - Anti-Vinculin antibody produced in rabbit, Vinculin Antibody Sigma
• MDL番号: MFCD00162905
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~116 kDa
• 化学種の反応性: rat, human, mouse
• 強化検証: independent; Learn more about Antibody Enhanced Validation
• テクニック: indirect immunofluorescence: 15-30 μg/mL using acetone-fixed frozen sections of rat or human heart; western blot: 2.5-5 μg/mL using whole extracts of cultured mouse NIH3T3 cells and human hepatoma HepG2 cells
• UniProtアクセッション番号: P18206
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... VCL(7414) ; mouse ... Vcl(22330) ; rat ... Vcl(305679)

詳細

詳細

In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletalmuscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.

免疫原

を介してKLHと結合した、ヒトビンキュリンのアミノ酸691~705 (N末端システイン付加) に相当する合成ペプチド。この配列はメタビンキュリンにも存在します。この配列はマウス、ラット、ブタと一致しており、ニワトリとは1アミノ酸異なっています。

アプリケーション

Anti-Vinculin antibody produced in rabbit has been used in immunoprecipitation.

Anti-vinculin antibody produced in rabbit has been used in following studies:

• immunofluorescence
• immuno-histology
• western blotting

生物化学的／生理学的作用

Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading. In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. Experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.

Vinculin also binds α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly. It transiently binds the actin-related protein-2/3 (ARP2/3) complex without colocalization in focal adhesions.

物理的形状

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable