SRP0195

PARP6 Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• 別名: MGC131971, Poly (ADP-ribose) Polymerase 6, pART17
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in baculovirus infected insect cells
• アッセイ: ≥70% (SDS-PAGE)
• 形状: aqueous solution
• 分子量: 98 kDa
• 包装: pkg of 10 μg
• 濃度: >0.02 mg/mL
• NCBIアクセッション番号: NM_020214
• UniProtアクセッション番号: Q2NL67
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... PARP6(56965)

詳細

詳細

PARP6 (poly(ADP-ribose) polymerase family member 6) gene is localized to human chromosome 15q23. It is a member of the family of multifunctional nuclear proteases known as PARPs, which contains 17 members and is further classified into three subtypes dependent on the catalytic domain structure. PARP6 is a type of mono(ADP-ribosyl) transferase on the basis that its catalytic domain has a significantly shorter nicotinamide-ribose-binding site than PARP1. This subfamily contains nine members including PARP6. PARP6 is composed of 630 residues and has a molecular weight of 71kDa.
Human PARP6 (GenBank Accession No. NM_020214), full length with N-terminal GST tag, MW = 98kDa, expressed in a Baculovirus infected Sf9 cell expression system.

アプリケーション

酵素キネティクスの研究をはじめ、インヒビターのスクリーニングや選択性プロファイリングにもご利用いただけます。

生物化学的／生理学的作用

PARP6 (poly(ADP-ribose) polymerase family member 6) negatively controls cell cycle, as its ectopic expression in HeLa cells leads to suppression of cell growth. It functions as a tumor suppressor in CRC (colorectal cancer), and its expression is associated with good prognosis. The ectopic expression of PARP6 results in suppressed expression of the oncogene survivin. In CRC PAPR6 prevents colony formation, invasion, migration, and cell proliferation.

単位の定義

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.

物理的形状

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注された希釈前の酵素を-70°Cで保存してください。注記:酵素は凍結解凍の繰り返しに非常に弱いので、注意してください。