SAE0023

Alanine Racemase from Bacillus stearothermophilus

buffered aqueous solution, ≥10 IU/mg solid

• 別名: Alanine Racemase from Bacillus stearothermophilus
• CAS番号: 9024-06-0
• Enzyme Commission number: 5.1.1.1 (BRENDA, IUBMB)
• MDL番号: MFCD03454300
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: Bacillus sp. (B. stearothermophilus)
• 品質水準: 200
• 形状: buffered aqueous solution
• 比活性: ≥10 IU/mg solid
• 分子量: M_r 78 kDa (2 subunits 39 kDa each)
• 保管温度: −20°C

詳細

アプリケーション

Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-¹⁴C]alanine to a racemic mixture of L/D- [¹⁴C]alanine †.

生物化学的／生理学的作用

Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†

単位の定義

1 unitは、L-アラニンデヒドロゲナーゼによる共役アッセイ系において、pH 10.5、30°C、1分間で、1.0 μmoleのD-アラニンをL-アラニンに変換します。

物理的形状

Aqueous solution containing 10 mM potassium phosphate

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable