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詳細
Phosphothreonine-BSA is a Phosphothreonine antibody inhibitor which blocks the reactivity of anti-phosphothreonine antibody. Antibodies against phosphorylated amino acid can be used as a tool for identification, quantification and immunoaffinity isolation of activated cellular proteins. The product specifically blocks the reactivity of anti-phosphothreonine antibody but does not block anti-phosphoserine- or anti-phosphotyrosine specific antibodies.
特異性
Working dilution is at least 10 μg/ml by the specific inhibition of the reactivity of Monoclonal Anti-Phosphothreonine (Sigma Product No. P 3555) in ELISA.
アプリケーション
Phosphothreonine-BSA can be used in various immunochemical assays like ELISA and immunoblotting for the specific inhibition of the reactivity of anti-phosphothreonine antibodies.
Phosphothreonine-BSA has been used in Pro-Q Diamond phosphoprotein gel staining method to detect phosphoamino acids.
生物化学的／生理学的作用
O-phospho-L-threonine conjugated to bovine serum albumin. Phosphothreonine antibody inhibitor specifically blocks the reactivity of anti-phosphothreonine antibody. It does not block the activity of anti-phosphotyrosine or anti-phosphoserine-specific antibodies.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
P3842-VAR:
P3842-.2ML:
P3842-BULK:
P3842-.2ML-PW:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Characterization of dynamic and steady-state protein phosphorylation using a fluorescent phosphoprotein gel stain and mass spectrometry
Schulenberg B, et al.
Electrophoresis, 25(15), 2526-2532 (2004)
Matthew G Vander Heiden et al.
Science (New York, N.Y.), 329(5998), 1492-1499 (2010-09-18)
Proliferating cells, including cancer cells, require altered metabolism to efficiently incorporate nutrients such as glucose into biomass. The M2 isoform of pyruvate kinase (PKM2) promotes the metabolism of glucose by aerobic glycolysis and contributes to anabolic metabolism. Paradoxically, decreased pyruvate
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