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Merck

GE28-9505-13

HiScreen SP Fast Flow

Cytiva 28-9505-13

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About This Item

UNSPSCコード:
47101511
NACRES:
NA.56

ligand

sulphopropyl

詳細

Ion Exchanger Type (value)

包装

pkg of 1 ea

メーカー/製品名

Cytiva 28-9505-13

パラメーター

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

ベッドサイズ

7.7 mm × 100 mm

ベッド容積

4.7 mL

カラム内径

7.7 mm

マトリックス

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

3-14

working range

4-13

適合性

suitable for bioprocess medium

詳細

HiScreen SP FF columns are prepacked with SP Sepharose Fast Flow, a strong cation exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

アプリケーション

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

特徴および利点

  • SP Sepharose Fast FLow is a strong cation exchanger and IEX Sepharose Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption.
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)
Store at 4 to 30 °C (20% Ethanol, 0.2 M Sodium Acetate)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

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資料

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

すべて表示

プロトコル

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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