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Merck

GE28-9440-06

Protein A Mag Sepharose

Cytiva 28-9440-06, pack of 1 × 500 μL

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About This Item

UNSPSCコード:
41106500
NACRES:
NA.56

ligand

native protein A

包装

pack of 1 × 500 μL

メーカー/製品名

Cytiva 28-9440-06

保管条件

(20% Ethanol, 20% medium slurry)

パラメーター

Room temperature temp. range

マトリックス

paramagnetic, spherical, highly cross-linked agarose particles

キャパシティ

8- 17 mg binding capacity(human IgG/ml gel)

保管温度

2-8°C

関連するカテゴリー

詳細

Protein A Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. Based on Sepharose with native Protein A as ligand.

アプリケーション

Protein A Mag Sepharose are magnetic beads designed to simplify enrichment of target Proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native Protein A as ligand. Protein A has high affinity for the Fc region of IgG antibodies from a broad variety of species.

There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis.

Protein A Mag Sepharose combines well-proven enrichment methods with the magnetic beads platform, which is highly suitable for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

特徴および利点

  • Visible and dense Sepharose based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
  • Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed
  • Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

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資料

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

This page describes immunoprecipitation (immunoaffinity or pull-down techniques) with GE Healthcare products.

プロトコル

This page shows how to separate IgG antibodies by affinity chromatography using Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra from GE Healthcare.

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