F0552

Fasリガンド マウス由来

>95% (SDS-PAGE), recombinant, expressed in mouse NSO cells, lyophilized powder

• MDL番号: MFCD00285792
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• リコンビナント: expressed in mouse NSO cells
• 品質水準: 100
• アッセイ: >95% (SDS-PAGE)
• 形状: lyophilized powder
• 分子量: monomer calculated mol wt ~18 kDa; 28-32 kDa by SDS-PAGE
• 不純物: endotoxin, tested
• UniProtアクセッション番号: P41047
• 保管温度: −20°C
• 遺伝子情報: mouse ... Fasl(14103)

詳細

詳細

FASLG (Fas ligand) acts as a ligand for Fas receptor, and is a major protein involved in programmed cell death, apoptosis. Soluble Fas (sFAS) is usually detected in plasma prior to apoptosis.

アプリケーション

Fas Ligand (FASLG) from mouse has been used for-

• the induction of apoptosis in PC12 cells and
• the induction of migration in BV-2 murine microglial cells.

生物化学的／生理学的作用

FASLG (Fas ligand) and Fas receptor constitute the basic elements in apoptosis. Interaction of FASLG with Fas receptor leads to activation of caspase-8. This caspase in turn leads to activation of effector caspases such as caspase-3, -6 and -7. This cascade results in the hydrolysis of nuclear and cytoplasmic components. Expression of FASLG is induced by nuclear factor-κB (NFκB). NFκB/FASLG pathway facilitates the suppression of p,p′-DDT (dichlorodiphenoxytrichloroethane)-induced cell toxicity by vitamin C and E. In CD4⁺ T cells, this protein is expressed on stimulus by T-cell receptor (TCR), both during normal and pathological conditions, such as alcohol exposure.

Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).

その他情報

マウスFASリガンド, アミノ酸残基132~279がコ-ドされています。N末端に6Xヒスチジンタグを付加しています。

物理的形状

凍結乾燥品(PBS溶液, 2.5 mg BSA, 0.2 μmフィルタ-減菌済み)。

アナリシスノート

ジャ-カット細胞のアポト-シス誘導能により活性を測定しています。

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable