b.End5

96091930, mouse brain, Endothelial-like

• 別名: Brain-derived Endothelial 5 Cells, bEnd3 Cells, bEnd5 Cells
• UNSPSCコード: 41106514

特性

• 製品名: b.End5, 96091930
• 由来生物: mouse brain
• 成長モード: Adherent
• 核型: Not specified
• 形態: Endothelial-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse Balb/c brain endothelioma

細胞株の説明

The cell line b.End5 has been established from brain endothelial cells of BALB/c mice. Immortalisation has been carried out by infection of primary cells with retrovirus coding for the Polyoma virus middle T-antigen. b.End5 cells are positive for endothelial specific proteins (PECAM-1, Endoglin, MECA-32, Flk-1) tested by FACS. Inflammatory cytokines induce the expression of proteins such as CAM-1, VCAM-1 and E-selectin. Injection into mice causes development of haemangiomas.

アプリケーション

Study of endothelial cell growth, differentiation and influence of oncogenes.

培地

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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