BLO-11

90112702

• UNSPSCコード: 41106514

特性

• 由来生物: mouse muscle
• 成長モード: Adherent
• 核型: 2n = 40
• 形態: Fibroblast-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse Moblo/y muscle hereditary emphysema

細胞株の説明

Established from the abdominal wall muscle of a 30 day old male Blotchy mouse (Mo (blo/y)) with hereditary emphysema. The cells are deficient in lysyl oxidase activity and accumulate high levels of intracellular copper. NOR-10 (Sigma Catalogue number. 90112701) was isolated from the abdominal wall muscle of a normal litter mate of the mouse from which BLO-11 was derived, thus it can serve as a matched control for studies with BLO fibroblasts.

アプリケーション

Genetic studies

培地

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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