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由来生物
bovine liver
品質水準
フォーム
ammonium sulfate suspension
比活性
≥6 U/mg protein
濃度
~8 U/mL
色
off-white
密度
1.2 g/mL at 20 °C
保管温度
2-8°C
アプリケーション
Dihydrofolate reductase (DHFR) is involved in the synthesis of purines, thymidine and glycine in folate metabolism. DHFR, from bovine liver, is used for methotrexate analysis . DHFR is useful to study various cancers such as sarcomas, leukemias and head and neck cancers .
生物化学的／生理学的作用
Dihydrofolate reductase (DHFR) reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as an electron donor. The binding of antitumor agents, such as methotrexate, to DHFR prevents the formation of reduced folates, which are essential for DNA synthesis .
単位の定義
1 unitは、pH 6.5、25°C、1分間で、1 μmolの7,8-ジヒドロ葉酸とNADPHを5,6,7,8-テトラヒドロ葉酸とNADPに変換する酵素量に相当します。
物理的形状
3.6 Mの硫酸アンモニウム溶液(pH 7.0)で調製した懸濁液
その他情報
In folate metabolism for the synthesis of purines, thymidine and glycine
Sales restrictions may apply
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves
Use of dihydrofolate reductase from bovine liver, rather than from L. casei, for determining methotrexate.
R J Brooks
Clinical chemistry, 24(3), 518-519 (1978-03-01)
M A Pesce et al.
Clinical chemistry, 27(3), 380-384 (1981-03-01)
Methotrexate was determined by the homogeneous enzyme immunoassay (EMIT) with the Multistat and CentrifiChem centrifugal analyzers and by the enzyme inhibition assay with use of the Multistat centrifugal analyzer. With both methods, the standard curve extends from 0.2 to 2.0
H B Eastman et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(19), 8572-8576 (1991-10-01)
Dihydrofolate reductase (DHFR; EC 1.5.1.3) is required in folate metabolism for the synthesis of purines, thymidine, and glycine. Although there have been several reports of induction of DHFR enzyme by methotrexate (MTX), a drug that competitively inhibits DHFR, there are
Luca Freschi et al.
Molecular bioSystems, 9(1), 36-43 (2012-10-27)
One of the most important challenges in systems biology is to understand how cells respond to genetic and environmental perturbations. Here we show that the yeast DHFR-PCA, coupled with high-resolution growth profiling (DHFR-qPCA), is a straightforward assay to study the
Inbar Avraham et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(52), E3678-E3686 (2012-12-01)
Plasmodium falciparum causes the deadliest form of human malaria. Its virulence is attributed to its ability to modify the infected RBC and to evade human immune attack through antigenic variation. Antigenic variation is achieved through tight regulation of antigenic switches
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