10109339001

Sorbitol Dehydrogenase (SDH)

from sheep liver

• 別名: SDH, Sorbitol Dehydrogenase, sorbitol
• Enzyme Commission number: 1.1.1.14 (BRENDA, IUBMB)
• UNSPSCコード: 12352204

特性

• 由来生物: sheep liver
• 品質水準: 100
• 形状: lyophilized
• 比活性: ~40 units/mg protein (At 25 °C with D-fructose as the substrate.)
• 分子量: 115 kDa
• 包装: pkg of 10 mg (60 mg lyophilizate)
• メーカー／製品名: Roche
• 濃度: 0.16 mg/mL Biuret
• テクニック: activity assay: suitable
• 色: white
• 最適pH: 7.5
• 溶解性: water: 60 mg/mL, clear, colorless
• 適合性: suitable for ELISA applications
• NCBIアクセッション番号: XP_004010700
• UniProtアクセッション番号: P07846
• アプリケーション: life science and biopharma
• その他の活性: ADH <0.00182%; GIDH <0.00152%; Glucose-DH <0.00182% (NAD as coenz.); LDH <0.00167%; MDH <0.00198%; NADH oxidase 0.00532%
• 保管温度: 2-8°C
• 遺伝子情報: sheep ... SORD(101115280)

詳細

詳細

L-iditol:NAD⁺ 5′-oxidoreductase
Sorbitol dehydrogenase (SDH) from sheep liver has a molar mass of 152kDa. The protein is a tetramer of four identical subunits. Each of these subunits has 355 amino acid residues, of which 10 are cysteine residues. Each subunit contains a zinc atom at the active site, which is associated with three protein ligands and a water molecule. The zinc atom associates with the oxygen of the sorbitol hydroxyl or of the fructose carbonyl interconverted during catalysis.

アプリケーション

Reduces L-iditol to L-sorbose. Also acts on D-glucitol and other closely related sugar alcohols. Allows the reduction of ketones to polyols (see aldolases for the synthesis of ketoses).

生物化学的／生理学的作用

Sorbitol dehydrogenase (SDH) catalyzes the reversible NAD-linked conversion of D-sorbitol to D-fructose and forms a part of the sorbitol pathway, which is a crucial bypass to glycolysis for glucose metabolism. This pathway has been found to be associated with the accumulation of sorbitol in lens, which results in diabetic cataractogenesis. SDH also oxidizes various polyols and other secondary alcohols into their corresponding ketones.

品質

Contaminants: <0.01% ADH, <0.02% GIDH and glucose dehydrogenase, each, <0.05% LDH and MDH, each.

単位の定義

One unit (U) sorbitol dehydrogenase will reduce 1 mol of D-fructose in one minute at +25 °C and pH 7.6 [triethanolamine buffer; 150 mM fructose (nonsaturating concentration)]. The above assay consumes 1 mol of NADH per mol of D-sorbitol formed.

物理的形状

Lyophilizate (60 mg contain 10 mg enzyme protein and 50 mg maltose).

調製ノート

Activator: The reactions (oxidation or reduction) are fastest in Tris or triethanolamine buffer.
Stabilizers: Maltose is used as stabilizer.
Storage conditions (working solution): An aqueous solution is stable at 2 to 8 °C for several weeks.

保管および安定性

Store at 2 to 8 °C. (Store the lyophilizate dry.)

その他情報

For life science research only. Not for use in diagnostic procedures.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): does not flash
• 引火点(℃): does not flash