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product name
PMC42-LA Human Breast Cancer Cell Line, PMC42-LA human breast cancer cell line is a mammary epithelial cell model that can be used to study epithelial to mesenchymal transition.
由来生物
human
品質水準
テクニック
cell culture | mammalian: suitable
詳細
Human breast carcinoma cell lines are useful models to investigate the pathogenesis of neoplastic transformation and aid in the development of novel therapeutic strategies. Nonetheless, there are few well characterized cell lines derived from human mammary carcinomas and loss of breast-specific marker expression and the emergence of dedifferentiated phenotypes over extended culture often limit their use .
Initiated from a pleural effusion of a metastatic breast cancer patient, the human mesenchymal breast carcinoma cell line PMC42 (also known as PMC42ET), is unique in that these cells remained pleomorphic even after several years of sustained culture . Typically grown in monolayer, PMC42 continue to give rise to numerous morphologically different cell types of both secretory and myoepithelial characteristics and this retained ability to differentiate in vitro earned their designation as stem cell like .
Initiated from a pleural effusion of a metastatic breast cancer patient, the human mesenchymal breast carcinoma cell line PMC42 (also known as PMC42ET), is unique in that these cells remained pleomorphic even after several years of sustained culture . Typically grown in monolayer, PMC42 continue to give rise to numerous morphologically different cell types of both secretory and myoepithelial characteristics and this retained ability to differentiate in vitro earned their designation as stem cell like .
PMC42-LA is a stable epithelial variant of the mesenchyme-like parental PMC42 /PMC42-ET cell line. PMC42-LA congregate into pavement epithelial sheets at high density, in which the cell-cell borders contain E-cadherin and -catenin, albeit 5-10% of cells do express the mesenchymal marker vimentin. When stimulated with epidermal growth factor (EGF), PMC42-LA cells undergo an epithelial-mesenchymal transition in which vimentin expression is upregulated and E-cadherin expression is reduced .
細胞株の説明
Cancer Cells
アプリケーション
Research Category
がん
腫瘍学
がん
腫瘍学
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
品質
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
保管および安定性
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SCC139:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
R H Whitehead et al.
Journal of the National Cancer Institute, 71(6), 1193-1203 (1983-12-01)
A new human breast carcinoma cell line (PMC42) has been further characterized. The cells can grow either as monolayers or as floating cords of cells. The cords grow in suspension for long periods but may spontaneously attach and grow out
Margaret L Ackland et al.
Laboratory investigation; a journal of technical methods and pathology, 83(3), 435-448 (2003-03-22)
PMC42-LA cells display an epithelial phenotype: the cells congregate into pavement epithelial sheets in which E-cadherin and beta-catenin are localized at cell-cell borders. They abundantly express cytokeratins, although 5% to 10% of the cells also express the mesenchymal marker vimentin.
L W Engel et al.
Cancer research, 38(11 Pt 2), 4327-4339 (1978-11-01)
A comprehensive listing of putative human breast carcinoma cell lines and the extent to which each has been characterized is presented. Criteria used to certify the human, mammary, and malignant origin of a cell line include: (a) a reliable histopathological
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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