おすすめの製品
由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
DO-7, monoclonal
形状
liquid
含まれません
preservative
化学種の反応性
bovine, monkey, human
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
avoid repeated freeze/thaw cycles
アイソタイプ
IgG2b
輸送温度
wet ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... TP53(7157)
詳細
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53.
Recognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue.
This Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-12) (Pantropic).
免疫原
Epitope: within amino acids 37-45 of p53
recombinant, human p53 expressed in E. coli
アプリケーション
Frozen Sections (1-2 g/ml)
Immunoblotting (1-2 g/ml)
Immunofluorescence (0.5 g/ml)
Immunoprecipitation (2 g/mg lysate)
Paraffin Sections (1-2 g/ml)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
アナリシスノート
Positive Control
SKBR-3 or MDA-231 cells or colon carcinoma tissue
SKBR-3 or MDA-231 cells or colon carcinoma tissue
その他情報
Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Lane, D.P. 1992. Nature358, 15.
Lane, D.P. 1992. Nature358, 15.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
11 - Combustible Solids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Jinichi Mori et al.
Neoplasia (New York, N.Y.), 19(3), 185-195 (2017-02-06)
p53 activation by cellular stresses induces the transcription of hundreds of its target genes. To elucidate the entire picture of its downstream pathway, we screened a cDNA microarray dataset of adriamycin-treated HCT116 p53-/- or p53+/+ cells and identified EPSIN 3
Jinichi Mori et al.
Cancer science, 107(3), 298-306 (2016-01-13)
In response to various cellular stresses, p53 is activated and inhibits malignant transformation through the transcriptional regulation of its target genes. However, the full picture of the p53 downstream pathway still remains to be elucidated. Here we identified cystatin C
Yuki Funauchi et al.
Scientific reports, 5, 16497-16497 (2015-11-13)
Accumulation of iron in tissues increases the risk of cancer, but iron regulatory mechanisms in cancer tissues are largely unknown. Here, we report that p53 regulates iron metabolism through the transcriptional regulation of ISCU (iron-sulfur cluster assembly enzyme), which encodes
Matthew T Balmer et al.
Cancer biology & therapy, 15(8), 1000-1012 (2014-05-08)
The chemotherapeutic agents doxorubicin (dox) or 5-fluorouracil (5FU) are used to treat cancer cells as they cause irreparable DNA damage, inducing these aberrant cells to undergo cell death. The mediator of this process is presumed to be in part the
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