MABS1118

Anti-Cdc4 (FBXW7) Antibody, clone 9D8.1

clone 9D8.1, from mouse

• 別名: F-box/WD repeat-containing protein 7, Archipelago, F-box and WD-40 domain protein 7, F-box protein FBW7, F-box protein FBX30, F-box protein SEL-10, hAgo, hCdc4, Homolog of C elegans sel-10
• UNSPSCコード: 12352203
• eCl@ss: 32160702

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 9D8.1, monoclonal
• 化学種の反応性: human, mouse
• テクニック: western blot: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NP_361014
• UniProtアクセッション番号: Q969H0
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FBXW7(55294)

詳細

詳細

F-box/WD repeat-containing protein 7 (UniProt Q969H0; also known as Archipelago, F-box and WD-40 domain protein 7, F-box protein FBW7, F-box protein FBX30, F-box protein SEL-10, hAgo, hCdc4, Homolog of C elegans sel-10) is encoded by the FBXW7 (also known as AGO, CDC4, FBW7, FBX30, SEL10) gene (Gene ID 55294) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. The F-box/WD repeat-containing protein 7 functions as an adaptor component of the Skp1, Cullin, F-box (SCF) and mediates substrates recruitment to the Cullin-RING E3 ubiquitin ligase (CRL) complex. Cyclin E, c-Myc, c-Jun, Notch1, SREBP, mTOR, c-Myb, MCL1, NF B2, and GATA3 are all known substrates targeted by Fbxw7 for ubiquitin-proteasome system- (UPS-) mediated degradation.

特異性

Clone 9D8.1 epitope lies within the N-terminal region specific to human and murine Cdc4 (FBXW7) spliced isoform 1 (Archipelago alpha, FBW7alpha, 110K, common), and not conserved in human/mouse isoform 2 (Archipelago beta, FBW7beta, 69K) or 3 (Archipelago gamma, FBW7gamma, Hippocampal). Note: UniProt Q8VBV4 currently lists only murine spliced isoform 3 (NM_080428), but not isoform 1 (NM_001177774) or 2 (NM_001177773).

免疫原

GST-tagged recombinant human Cdc4 (FBXW7) isoform 1 (Archipelago alpha, FBW7alpha) N-terminal fragment.

アプリケーション

Research Category
細胞シグナル伝達

Detect Cdc4 (FBXW7) using this mouse monoclonal Anti-Cdc4 (FBXW7) Antibody, clone 9D8.1, Cat. No. MABS1118, validated for use in Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cdc4 (FBXW7) in 10 µg of NIH/3T3, HeLa, HepG2, K562, LNCaP, U2OS nuclear extracts.

品質

Evaluated by Western Blotting in HEK293 nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cdc4 (FBXW7) in 10 µg of HEK293 nuclear extract.

ターゲットの説明

~80 kDa observed. 79.66 kDa (human; UniProt Q969H0-1) and mouse; NP_001171244 & NM_001177774) calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified.

Format: Purified

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable