MABN2322

Anti-Nerve Growth Factor Antibody, clone α D11

• 別名: NGF;beta-NGF
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: rat
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: α D11, monoclonal
• 分子量: calculated mol wt 27.088 kDa
• 精製方法: using protein G
• 化学種の反応性: chicken, human, mouse
• 包装: antibody small pack of 100 μg
• テクニック: ELISA: suitable
• アイソタイプ: IgG2a
• エピトープ配列: N-terminal half
• タンパク質IDアクセッション番号: NP_001106168
• UniProtアクセッション番号: P01139
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Ngf(18049)

詳細

詳細

Beta-nerve growth factor (UniProt: P01139; also known as Beta-NGF) is encoded by the Ngf (also known as Ngfb) gene (Gene ID: 18049) in murine species. β-NGF, an endogenous neurotrophic factor, is a homodimeric glycoprotein that is synthesized with a signal peptide (aa 1-18) and a propeptide (aa 19-121) that are subsequently cleaved off to generate the mature form (aa 122-241). It is highly expressed in male submaxillary gland and its levels are much lower in female submaxillary gland. Its expression is reported to oscillate in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain. NGF is important for the development and maintenance of the sympathetic and sensory nervous systems. It serves as an extracellular ligand for the NTRK1 and NGF receptors to activate cellular signaling cascades to regulate neuronal proliferation, differentiation, and survival. However, it does not permeate the blood-brain barrier. Its precursor form (ProNGF) is reported to function as a ligand for the heterodimeric receptor formed by Sortilin Related VPS10 Domain Containing Receptor 2 (SORCS2) and NGF receptor that activates signaling cascade leading to inactivation of RAC1 and/or RAC2. In contrast to the mature form, ProNGF is known to promote neuronal apoptosis. N-glycosylation of ProNGF is reported to be essential for its processing, secretion, and construction of the tertiary fold of the β-NGF homodimer. Clone D11 is a potent antagonist that can effectively antagonize rodent and human NGF in a variety of in vitro and in vivo systems. (Ref.: Covaceuszach, S., et al. (2012). PLoS ONE 7(3): e32212; Feng, D., et al. (2010). J. Mol. Biol. 396(4); 967-984; Scott, J., et al. (1983). Nature. 302(5908); 538-540).

特異性

Clone D11 is a rat monoclonal antibody that detects Nerve growth factor (NGF). It targets an epitope within 23 amino acids from the N-terminal half of the mature NGF.

免疫原

2.5S NGF purified from the mouse submandibular glands.

アプリケーション

Quality Control Testing

Evaluated by ELISA with recombinant mouse Nerve growth factor.

ELISA Analysis (ELISA): A representative lot of this antibody detected various dilution of recombinant mouse Nerve growth factor.

Tested applications

Surface plasmon resonance: A representative lot detected Nerve Growth Factor in Surface plasmon resonance applications (Covaceuszach, S., et al. (2012). PLoS One. 7(3):e32212).

ELISA Analysis: A representative lot detected Nerve Growth Factor in ELISA applications (Cattaneo, A., et al. (1988). J. Neurochem. 50(4):1003-10; Covaceuszach, S., et al. (2012). PLoS One. 7(3):e32212).

Inhibition Assay: A representative lot competitively inhibited the biological activity of mNGF and prevented the binding of NGF to PC12 cells. (Cattaneo, A., et al. (1988). J. Neurochem. 50(4):1003-10).

Radioimmunoassay: A representative lot detected Nerve growth factor in Radioimmunoassay applications (Cattaneo, A., et al. (1988). J. Neurochem. 50(4):1003-10).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Nerve Growth Factor, clone D11, Cat. No. MABN2322, is a rat monoclonal antibody that detects Nerve growth factor and is tested for use in ELISA, Inhibition Assay, Radioimmunoassay, and Surface Plasmon Resonance.

物理的形状

Purified rat monoclonal antibody IgG2a in PBS without preservatives.

保管および安定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable