MABF981

Anti-CXCR4 Antibody, clone 12G5

clone 12G5, from mouse

• 別名: C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, LESTR, Lipopolysaccharide-associated protein 3, LAP-3, LPS-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, SDF-
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 12G5, monoclonal
• 化学種の反応性: human
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable; neutralization: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_001008540
• UniProtアクセッション番号: P61073
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CXCR4(7852)

詳細

詳細

C-X-C chemokine receptor type 4 (UniProt P61073; also known as CD184, CXC-R4, CXCR-4, FB22, Fusin, HM89, LAP-3, LCR1, LESTR, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3, LPS-associated protein 3, Neuropeptide Y receptor Y3, NPYRL, SDF-1 receptor, Stromal cell-derived factor 1 receptor) is encoded by the CXCR4 (also known as D2S201E, HSY3RR, LAP3, NPY3R, NPYR, NPYY3R, WHIM, WHIMS) gene (Gene ID 7592) in human. Chemokines and their receptors direct migration of distinct leukocyte subsets to sites of inflammation and to their specific niches in lymphoid organs. Virtually all T cell chemoattractants selectively attract memory/activated T cells. CXCR4 is the seven-transmembrane receptor for SDF-1 that is essential for the development of non-hematopoietic tissues, including heart and B lymphocytes. CXCR4 and CCR5 are two chemokine receptors that also function as co-receptors for human immunodeficiency virus (HIV) entry into CD4+ cells. During the early stages of HIV infection, viral isolates tend to use CCR5 for viral entry, while later isolates tend to use CXCR4.

免疫原

Epitope: Extracellular domain

Simian immunodeficiency virus (SIVmac) variant CP-MAC-infected Sup-T1 human T lymphoblasts.

アプリケーション

Research Category
炎症及び免疫

Research Sub Category
免疫グロブリン及び免疫

Flow Cytometry Analysis: A representative lot detected surface expression of human CXCR4 in various cell types by flow cytometry (Bleul, C.C., et al. (1997). Proc. Natl. Acad. Sci. U S A.94(5):1925-1930; McKnight, A., et al. (1997). J. Virol. 71(2):1692-1696; Endres, M.J., et al. (1996). Cell. 87(4):745-756)
Immunocytochemistry Analysis: A representative lot stained paraformaldehyde-fixed CHO cells transfected to stably express human CXCR4/fusin (Endres, M.J., et al. (1996). Cell. 87(4):745-756).
Neutralizing Analysis: A representative lot blocked SDF-1-induced lymphocyte chemotaxis using freshly isolated human PBMCs (Bleul, C.C., et al. (1997). Proc. Natl. Acad. Sci. U S A.94(5):1925-1930).
Neutralizing Analysis: A representative lot blocked the infection of CXCR4+/CD4+ rhabdomyosarcoma (RD) cells by seven HIV-1 (LAI, RF, Gun-1wt, Gun-1var) and HIV-2 (ROD A/B and CBL-23) strains as assessed by >95% reduced syncytium (multinucleated cell) formation due to viral infection-induced cell-to-cell fusion. Clone 12G5 also blocked Gun-1wt, Gun-1var, and CBL-23, but not the other 4 HIV strains from infecting HeLa/CD4 and C8166 cultures (McKnight, A., et al. (1997). J. Virol. 71(2):1692-1696).
Neutralizing Analysis: A representative lot blocked HIV-2 /vcp strain from infecting U87 cells transfected to express human CXCR4/fusin as assessed a reduction of syncytium (multinucleated cell) formation due to viral infection-induced cell-to-cell fusion (Endres, M.J., et al. (1996). Cell. 87(4):745-756).
Note: Although clone 12G5 binds cell surface CXCR4, efforts to immunoprecipitate or immunoblot CXCR4 were reported to be unsuccessful (Endres, M.J., et al. (1996). Cell. 87(4):745-756).

This Anti-CXCR4 Antibody, clone 12G5 is validated for use in Flow Cytometry, Immunocytochemistry and Neutralizing for the detection of CXCR4.

品質

Evaluated by Flow Cytometry in Jurkat cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CXCR4 in Jurkat cells.

ターゲットの説明

~39 kDa calculated

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in PBS without preservatives.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable