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由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
20G2C12, monoclonal
分子量
calculated mol wt 21.59 kDa
observed mol wt ~25 kDa
化学種の反応性
human
包装
antibody small pack of 100 μL
テクニック
co-immunoprecipitation (co-IP): suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG1κ
UniProtアクセッション番号
輸送温度
dry ice
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... TFAM(7019)
詳細
Transcription factor A, mitochondrial (UniProt: Q00059; also known as mtTFA, Mitochondrial transcription factor 1, MtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2, TFAM) is encoded by the TFAM (also known as TCF6, TCF6L2) gene (Gene ID: 7019) in human. TFAM is a DNA binding protein that plays multiple roles in regulating mitochondrial DNA function. As a sequence-specific transcription factor, it binds upstream of the light strand promoter and heavy strand promoter 1 to activate initiation of transcription. It is a component of the mitochondrial transcription initiation complex (composed of TFB2M, TFAM and POLRMT). In this complex, TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. TFAM contains two HMG boxes (aa 50-118 and 155-219). Phosphorylation by protein kinase A within the HMG box 1 is shown to impair its DNA binding and promotes its degradation by AAA+ Lon protease. Mutations in TFAM gene are known to cause hepatocerebral type of mitochondrial DNA depletion syndrome that is characterized by severe intrauterine growth restriction, neonatal-onset hypoglycemia and liver dysfunction. In this disease hepatic pathology includes cirrhosis, steatosis and cholestasis that leads to liver failure. (Ref.: Wang, YE., et al. (2013). PLoS ONE. 8(8); e74513).
特異性
Clone 20G2C12 is a mouse monoclonal antibody that detects human mitochondrial Transcription Factor A (TFAM).
免疫原
Full-length recombinant human Transcription factor A, mitochondrial (TFAM).
アプリケーション
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected TFAM in HeLa cell lysates.
Tested Applications
ChIP-seq Analysis: A representative lot detected TFAM in ChIP-seq applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TFAM in Immunoprecipitation applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TFAM in HeLa cells.
Western Blotting Analysis: A representative lot detected TFAM in Western Blotting applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunocytochemistry Analysis: A representative lot detected TFAM in Immunocytochemistry applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HeLa cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected TFAM in HeLa cell lysates.
Tested Applications
ChIP-seq Analysis: A representative lot detected TFAM in ChIP-seq applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TFAM in Immunoprecipitation applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TFAM in HeLa cells.
Western Blotting Analysis: A representative lot detected TFAM in Western Blotting applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunocytochemistry Analysis: A representative lot detected TFAM in Immunocytochemistry applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-TFAM, clone 20G2C12, Cat. No. MABE1994, is a mouse monoclonal antibody that detects Transcription factor A, mitochondrial (TFAM) and is tested for use in ChIP-seq, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
物理的形状
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at +2°C to +8°C from date of receipt.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABE1994-25UL:
MABE1994-100UL:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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