MABE121

Anti-SMARCA4 Antibody, clone 20C3.2

clone 20C3.2, from mouse

• 別名: Transcription activator BRG1, ATP-dependent helicase SMARCA4, BRG1-associated factor 190A, BAF190A, Mitotic growth and transcription activator, Protein BRG-1, Protein brahma homolog 1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regulato
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 20C3.2, monoclonal
• 化学種の反応性: rat, human
• テクニック: immunocytochemistry: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_001122316
• UniProtアクセッション番号: P51532
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SMARCA4(6597)

詳細

詳細

Also known as Protein BRG1 and SNF2-beta, among other aliases, SMARCA4 a transcriptional activator belonging to the SNF2/RAD54 helicase family, and acts as a component of the SWI/SNF ATP-dependent chromatin remodeling complexes family. BRG1 contains 4 domains of interest; a bromo domain, a helicase ATP-binding domain, a helicase C-terminal domain, and a HAS domain. It is a component of several different complexes with distinctive roles such as the CREST-BRG1 complex which regulates the activation of promoters in a calcium-dependent manner, releasing repressor complexes and recruiting activator complexes. It is also a part of the nBAF and npBAF complexes which are involved in the chromatin remodeling of mononucleosomes and nucleosomal arrays. As a component of the SWI/SNF complex, SMARCA4 has a role in tumor suppression and has been shown to regulate cell cycle and differentiation when present in neoplasma.

免疫原

GST-tagged recombinant protein corresponding to the human SMARCA4.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
エピジェネティクス

Anti-SMARCA4 Antibody, clone 20C3.2 is a Mouse Monoclonal Antibody for detection of SMARCA4 also known as Transcription activator BRG1, SNF2-beta & has been validated in WB, ICC.

Western Blot Analysis: 0.5 µg/mL of this antibody detected SMARCA4 in PC-12 cell lysate.

Immunocytochemistry Analysis: 1 µg/mL of this antibody detected SMARCA4 in HeLa and A431 cells.

品質

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 0.5 µg/mL of this antibody detected SMARCA4 in 10 µg of HeLa nuclear extract.

ターゲットの説明

~184 kDa observed

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
HeLa nuclear extract

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable