おすすめの製品
由来生物
mouse
品質水準
抗体製品の状態
ascites fluid
抗体製品タイプ
primary antibodies
クローン
10C8, monoclonal
化学種の反応性
human, mouse
テクニック
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... WNT1(7471)
関連するカテゴリー
詳細
Wnt-1, also known as Proto-oncogene Wnt-1 or Proto-oncogene Int-1 homolog, was first identified as a proto-oncogene activated by viral insertion in mouse mammary tumors. The Wnt-1 gene encodes a member of a large family of secreted proteins that are cysteine-rich, glycosylated, and poorly soluble. Wnt-1 serves as a ligand for the RYK coreceptor, resulting in Wnt signaling. The Wnt signalling pathway mediates the levels of cytosolic beta-catenin, a multi-functional protein that associates with membrane-bound E-cadherin as well as several DNA-binding proteins, such as members of the TCF/LEF family. Additionally, it serves a ligand for members of the frizzled family of seven transmembrane receptors and plays a role in development of the central nervous system.
免疫原
Purified recombinant fragment of human WNT1 expressed in E. Coli.
アプリケーション
Research Category
幹細胞研究
幹細胞研究
Research Sub Category
多能性及び初期分化
多能性及び初期分化
Detect Wnt-1 using this mouse monoclonal antibody, Anti-WNT1 Antibody, clone 10C8 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WNT1 in HeLa and 3T3-L1 cells.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected WNT1 in human adrenal tissue.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected WNT1 in HeLa cells.
Optimal working dilutions must be determined by end user.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected WNT1 in human adrenal tissue.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected WNT1 in HeLa cells.
Optimal working dilutions must be determined by end user.
品質
Evaluated by Western Blotting in NIH/3T3, 3T3L1, and HeLa cell lysates.
Western Blot Analysis: A 1:500-2,000 dilution of this antibody detected WNT1 in NIH/3T3, 3T3L1, and HeLa cell lysates.
Western Blot Analysis: A 1:500-2,000 dilution of this antibody detected WNT1 in NIH/3T3, 3T3L1, and HeLa cell lysates.
ターゲットの説明
~41 kDa observed. Uncharacterized bands may appear in some lysate(s).
物理的形状
Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
NIH/3T3, 3T3L1, and HeLa cell lysates
NIH/3T3, 3T3L1, and HeLa cell lysates
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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