MABD129

Anti-PRDM1 Antibody, clone 5E7

ascites fluid, clone 5E7, from mouse

• 別名: PR domain zinc finger protein 1, BLIMP-1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1, Positive regulatory domain I-binding factor 1, PRDI-BF1, PRDI-binding factor 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: 5E7, monoclonal
• 化学種の反応性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG1
• UniProtアクセッション番号: O75626
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PRDM1(639)

詳細

詳細

PRDM1/Blimp1 is a repressor of beta-interferon gene expression. In the nucleus, PRDM1 is known to bind specifically with the Beta-interferon gene positive regulatory domain I (PRDI), and to interact with PRMT5. During B-cell differentiation, PRDM1 actively represses the transcription of the gene, c-myc, through binding its promoter and has been deemed a “master regulator” for terminal B-cell differentiation, as it is able to initiate the BCL1 cell developmental cascade. The transcription of this gene is increased upon virus infection.

免疫原

Purified recombinant fragment of human PRDM1 expressed in E. Coli.

アプリケーション

Research Category
幹細胞研究

Research Sub Category
多能性及び初期分化

Detect PRDM1 using this mouse monoclonal antibody, Anti-PRDM1 Antibody & clone 5E7 validated for use in western blotting.

品質

Evaluated by Western Blotting in Raji, L1210, and TPH-1 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PRDM1 in Raji, L1210, and TPH-1 cell lysates.

Optimal working dilutions must be determined by end user.

ターゲットの説明

~88 kDa observed. Uncharacterized bands may appear in some lysate(s).

物理的形状

Unpurified

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

アナリシスノート

Control
Raji, L1210, and TPH-1 cell lysates

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable