MAB374-AF488

Anti-GAPDH Antibody, clone 6C5, Alexa Fluor^™ 488 conjugate

clone 6C5, from mouse, ALEXA FLUOR^™ 488

• 別名: Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: ALEXA FLUOR^™ 488
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 6C5, monoclonal
• 化学種の反応性: mouse, human
• 化学種の反応性（ホモロジーによる予測）: cat (based on 100% sequence homology), fish (based on 100% sequence homology), canine (based on 100% sequence homology), porcine (based on 100% sequence homology), rat (based on 100% sequence homology), rabbit (based on 100% sequence homology)
• テクニック: immunocytochemistry: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NM_001082253.1
• UniProtアクセッション番号: P46406
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GAPDH(2597) ; mouse ... Gapdh(14433) ; rabbit ... Gapdh(100009074)

詳細

詳細

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Its binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic role in metabolism, it is also involved in the initial stages of apoptosis or oxidative stress response where it is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH can also bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders, including the beta-amyloid precursor protein and the Huntingtin protein where reduced function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity.

特異性

It has been reported that this antibody does not react with GAPDH from E. coli.

アプリケーション

This Anti-GAPDH Antibody, clone 6C5, Alexa Fluor^™ 488 conjugate is validated for use in Immunocytochemistry for the detection of GAPDH.

品質

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GAPDH in A431 cells.

ターゲットの説明

~38 kDa observed

物理的形状

Purified mouse conjugate antibody in buffer containing PBS 15 mg/ml BSA and 0.1% Sodium Azide.

その他情報

Concentration: Please refer to lot specific datasheet.

法的情報

ALEXA FLUOR is a trademark of Life Technologies

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable