MAB079-1

Anti-S-100 Protein Antibody, clone 15E2E2

clone 15E2E2, Chemicon^®, from mouse

• 別名: Calcium Binding Protein A1/B1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 15E2E2, monoclonal
• 化学種の反応性: bovine, rat, human, mouse
• メーカー／製品名: Chemicon^®
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NM_006271.1
• UniProtアクセッション番号: P23297
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... S100A1(6271)

詳細

詳細

S-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.

特異性

This antibody recognizes ~22 kDa (non-reduced) or 11 kDa (reduced) of S100. It reacts with human, rat, mouse, and cow (bovine). Other species are not tested.

免疫原

Epitope: Recognizes both alpha & beta S100

Purified bovine S-100 protein

アプリケーション

Research Category
代謝

Research Sub Category
筋生理学

Anti-S-100 Protein Antibody, clone 15E2E2 is an antibody against S-100 Protein for use in IH, IH(P), IP & WB.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.

Immunohistochemistry:
A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.

Optimal working dilutions must be determined by end user.

品質

Evaluated by Western Blot on PC12 lysates

Western Blot Analysis:
1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate.

物理的形状

Protein A purified

Format: Purified

This affinity purified antibody is supplied in sterile phosphate buffered saline (pH 7.2) containing antibody stabilizer. Contains 0.01% sodium azide.

保管および安定性

Stable for 1 year at 2-8ºC from date of receipt.

アナリシスノート

Control
Positive Control Tissue: Melanoma

その他情報

Concentration: 0.5 mg/mL

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable