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由来生物
rabbit
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性（ホモロジーによる予測）
all
テクニック
western blot: suitable
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
詳細
2A peptides and 2A-like peptide sequences (also known as CHYSEL, or cis-acting hydrolase elements) are a superior alternative to internal ribosomal entry sites (IRES) for coordinating the expression of multiple gene products from a single recombinant construct. 2A peptides allow multiple proteins to be encoded as polyproteins, which then dissociate into component proteins upon translation. The 2A sequence impairs normal peptide bond formation via a mechanism called ribosomal skipping, resulting in effective, non-enzymatic generation of distinct peptide products from a single multicistronic construct. The use of 2A sequences allows the stoichiometric production of up to four proteins from a single vector, making it a powerful tool for the equimolar expression of multiple cistrons.
特異性
Recognizes the 2A sequence, derived from Foot and Mouth picornavirus (VKQTLNFDLLKLAGDVESNPG*P), where * represents the 2A cleavage site. The epitope is believed to be within the “GDVESNPG” region since the antibody also recognizes the unrelated 2A regions from Thosea asigna virus and Equine rhinitis A virus.
免疫原
Epitope: The epitope is believed to be within the “GDVESNPG” region.
The immunogen used was recombinant 2A from foot and mouth virus1.
アプリケーション
Research Category
エピトープタグ抗体及び凡用抗体
エピトープタグ抗体及び凡用抗体
Research Sub Category
エピトープタグ
エピトープタグ
Anti-2A Peptide Antibody is an antibody against 2A Peptide for use in WB.
品質
Western Blot Analysis:
A multicistronic vector was synthesized consisting of several CD3 proteins linked with 2A peptide sequences from foot and mouth disease, Thosea asigna, and Equine rhinitis A viruses, respectively (CD3δ-F2A-CD3ε-T2A-CD3γ-E2A-CD3ζ)1. The vector was transduced in 293 cells. A 1:1000 dilution of this antibody detected the 2A sequence in all three CD3-2A proteins (CD3 epsilon, gamma, delta) generated from a single 2A peptide-linked retroviral vector.
A multicistronic vector was synthesized consisting of several CD3 proteins linked with 2A peptide sequences from foot and mouth disease, Thosea asigna, and Equine rhinitis A viruses, respectively (CD3δ-F2A-CD3ε-T2A-CD3γ-E2A-CD3ζ)1. The vector was transduced in 293 cells. A 1:1000 dilution of this antibody detected the 2A sequence in all three CD3-2A proteins (CD3 epsilon, gamma, delta) generated from a single 2A peptide-linked retroviral vector.
ターゲットの説明
varies
関連事項
Replaces: 09-085
物理的形状
Serum with 0.05% NaN3.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
2A-containing recombinant proteins
2A-containing recombinant proteins
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABS31:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Processing of the VP1/2A junction is not necessary for production of foot-and-mouth disease virus empty capsids and infectious viruses: characterization of "self-tagged" particles.
Gullberg, M; Polacek, C; B?tner, A; Belsham, GJ
Journal of virology null
Xiang Jiao et al.
RSC advances, 8(61), 34967-34972 (2018-10-12)
The yeast Saccharomyces cerevisiae serves as a promising host for the production of a wide range of chemical compounds and fuels. Currently, simultaneous expression of several genes could be achieved via the use of 2A viral peptides, yet detailed characterizations
Thea Kristensen et al.
PLoS pathogens, 15(1), e1007509-e1007509 (2019-01-19)
Many picornaviruses cause important diseases in humans and other animals including poliovirus, rhinoviruses (causing the common cold) and foot-and-mouth disease virus (FMDV). These small, non-enveloped viruses comprise a positive-stranded RNA genome (ca. 7-9 kb) enclosed within a protein shell composed
Lasse Neukirch et al.
Oncotarget, 10(14), 1458-1472 (2019-03-13)
Endogenous retroviruses (ERVs) that make up 8% of the human genome have been associated with the development and progression of cancer. The murine model system of the melanoma associated retrovirus (MelARV), which is expressed in different murine cancer cell lines
Alexandra Merkx-Jacques et al.
Biotechnology for biofuels, 11, 248-248 (2018-09-22)
Thraustochytrids are heterotrophic, oleaginous, marine protists with a significant potential for biofuel production. High-value co-products can off-set production costs; however, the cost of raw materials, and in particular carbon, is a major challenge to developing an economical viable production process. The
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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