ABC112

Anti-phospho-ULK1/ATG1 (Ser758) Antibody

from rabbit, purified by affinity chromatography

• 別名: Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, ATG1, hATG1, Unc-51-like kinase 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: bovine (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• テクニック: inhibition assay: suitable (peptide); western blot: suitable
• NCBIアクセッション番号: NP_003556
• UniProtアクセッション番号: O75385
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pSer758)
• 遺伝子情報: human ... ULK1(8408)

詳細

詳細

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.

特異性

This antibody recognizes the ULK1/ATG1 phosphorylated at Ser758.

免疫原

Epitope: Phosphorylated Ser758

KLH-conjugated linear peptide corresponding to human ULK1/ATG1 phosphorylated at Ser758.

アプリケーション

Research Category
アポトーシス及び癌

Research Sub Category
アポトーシス-追加

Detect phospho-ULK1/ATG1 (Ser758) using this Anti-phospho-ULK1/ATG1 (Ser758) Antibody validated for use in Western Blotting, Peptide Inhibition Assay.

Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot blocked phospho-ULK1/ATG1 (Ser758) in serum-starved A431 cell lysates.

品質

Evaluated by Western Blot in A431 and serum starved A431 cell lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected ULK1/ATG1 on 10 µg of A431 and serum starved A431 cell lysates..

ターゲットの説明

~120 kDa observed

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
A431 and serum starved A431 cell lysates.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable