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由来生物
mouse
品質水準
結合体
CY5 conjugate
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
monoclonal
化学種の反応性
vertebrates
メーカー／製品名
Upstate®
テクニック
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
アイソタイプ
IgG1
輸送温度
wet ice
ターゲットの翻訳後修飾
phosphorylation (pSer/pThr)
詳細
This antibody recognizes a variety of proteins that are phosphorylated during mitosis.
Progression of cells from interphase to mitosis involves alterations in cell structures and activities. The transition from G2 to M phase is induced by M phase-promoting factor, or MPF. In M phase, many proteins are phosphorylated directly by MPF or indirectly by kinases activated by MPF. These M-phase phosphoproteins (MPPs, or MPHOSPHs) permit disassembly of interphase structures and generation of M-phase enzymatic activities and structures. Also, known as Forkhead-related protein FKHL16 or Hepatocyte nuclear factor 3 forkhead homolog 11.
特異性
Recognizes a phosphorylated epitope (phospho-[Ser/Thr]Pro) found in phosphoproteins such as MAP2, HSP70, cdc25, and DNA topoisomerase IIα, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM-2 varies from species to species and with the cell type.
免疫原
Mitotic human HeLa cell lysate
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
細胞周期、DNA複製及び修復
細胞周期、DNA複製及び修復
Anti-phospho-Ser/Thr-Pro Antibody, MPM-2, Cy5 conjugate is a Mouse Monoclonal Antibody for detection of phospho-Ser/Thr-Pro also known as Mitotic protein #2 & has been tested in IF, WB, ICC.
品質
Routinely evaluated by immunoblot on colcemid-treated HeLa cell lysates.
物理的形状
Protein G Purified
100μg Cy5-conjugated mouse IgG1 in 200μl of PBS containing 1% BSA, 0.05% Tween®-20, 0.05% sodium azide.
保管および安定性
1 year at 4°C from date of shipment
アナリシスノート
Control
Colcemid-treated HeLa cell lysates
Colcemid-treated HeLa cell lysates
法的情報
TWEEN is a registered trademark of Croda International PLC
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
16-220:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Thomas Blasi et al.
Nature communications, 7, 10256-10256 (2016-01-08)
Imaging flow cytometry combines the high-throughput capabilities of conventional flow cytometry with single-cell imaging. Here we demonstrate label-free prediction of DNA content and quantification of the mitotic cell cycle phases by applying supervised machine learning to morphological features extracted from
Nicola Brownlow et al.
Nature communications, 5, 5685-5685 (2014-12-09)
Exit from mitosis is controlled by silencing of the spindle assembly checkpoint (SAC). It is important that preceding exit, all sister chromatid pairs are correctly bioriented, and that residual catenation is resolved, permitting complete sister chromatid separation in the ensuing
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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