HomeSolid Phase Microextraction (SPME)Bioanalytical Applications for SPME

Bioanalytical Applications for SPME

Key Features and Benefits

  • Direct sampling of biofluids
  • Easily automated for fast method development
  • Suitable for LC-MS, DESI, DART, and other systems

Figure 1.

Technology Overview

BioSPME Tips, based on SPME, is a fast platform which allows for 1-step bioanalytical LC/MS sample handling (integrated sampling and sample preparation steps), providing the highest throughput of any bioanalytical sample handling technique to date. BioSPME Tips enable direct sampling of biofluids, eliminating the need for time-consuming sample preparation steps used in current bioanalytical analyses. BioSPME Tips are suitable for use with all leading robotics capable of 96-well plate technology for easy automated method development. They are also available in several chemistries, offering unique selectivity for a variety of compound classes; including amphetamines and synthetic amphetamines.


Figure 2.Linearity of Extractions from 100 µL Samples from Rat Plasma with Detection Greater than 10 ng/mL

Recommended Reading

Title: Bioanalysis with SPME
Authors: H. Lord, E. Cudjoe, D. Vuckovic, P. Togunde, F.M. Musteata, S.N. Zhou, X. Zhang, Md E. Hoque, J. Pawliszyn
Affiliation: University of Waterloo, Waterloo, ON, Canada
Publication: Reporter, Volume 29.2

Title: C18 SPME Fiber Probes for LC Applications
Authors: Robert Shirey, Craig Aurand
Affiliation: Supelco®/Sigma-Aldrich®
Publication: Reporter, Volume 28.3

Title: Solid phase microextraction. How far are we from clinical practice?
Authors: Barbara Bojko, Erasmus Cudjoe, Marcin Wasowicz, Janusz Pawliszyn
Affiliation: University of Waterloo
Journal: Trends in Analytical Chemistry, Vol. 30, No. 9, 2011

Title: Integration and miniaturization facilitate on-site and in-vivo analysis
Author: Janusz Pawliszyn
Affiliation: University of Waterloo
Journal: Trends in Analytical Chemistry, Vol. 30, No. 9, 2011

Title: Coupling Desorption Electrospray Ionization with Solid Phase Microextraction for Screening and Quantitative Analysis of Drugs in Urine
Authors: Joseph H. Kennedy,† Craig Aurand,‡ Robert Shirey,‡ Brian C. Laughlin,† and Justin M. Wiseman,†
Affiliation: † Prosolia, Inc., Indianapolis, Indiana; ‡ Supelco®/Sigma-Aldrich®, Bellefonte, Pennsylvania
Journal: Anal. Chem. 2010, 82, 7502-7508

Title: Biocompatible Solid Phase Microextraction Coatings and Methods for their Preparation
Authors: Janusz Pawliszyn, et al.
Affiliation: University of Waterloo
Journal: United States Patent Application Publication, 2009/0026122 A1