HLB and Ion-Exchange phases for a wide range of applications and pH conditions

Supel-Select SPE phases are ideal for the solid phase extraction of a broad range of compounds from aqueous samples. While reversed-phase interactions dominate retention on the Supel-Select HLB, and the retention mechanisms of the Supel-Select SAX and SCX are predominately based on ion-exchange, the hydrophilic modifications of the styrene-based polymer backbone allow for retention and recovery of more polar compounds.

Table 1.
Table 2.

High and Reproducible Recoveries

The hydrophilic, lipophilic balanced Supel-Select HLB SPE allows users to extract a broad range of compounds using a single sorbent and generic methodology. Analyte recovery was high across all the compounds tested, and results were highly reproducible across three production lots.

Supel-Select HLB SPE

Minimal Extractables

Supel-Select HLB SPE phases are quality controlled for both LC-UV and LC-MS/MS extractables. As assays require greater sensitivity, analytical “cleanliness” of both SPE phases and associated hardware becomes more critical.
In this simple test, acetonitrile was passed through both Supel-Select HLB SPE tubes and the leading competitor’s tubes followed by LC-UV analysis.

Supel-Select HLB SPE imparted less than 25% of UV extractables than the leading competitor.

Supel-Select HLB VS

Figure 1.