Appendix 4 extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007

Simple IEX chromatography, such as elution by a step-gradient, can be performed using a syringe or peristaltic pump with prepacked HiTrap columns. A chromatography system is required in order to achieve a high resolution separation using accurately controlled linear gradient elution, to take advantage of the high flow rates of modern media, or when the same column is to be used for many runs.

Standard ÄKTAdesign configurations
Standard ÄKTAdesign configurations

Figure 1. Standard ÄKTAdesign configurations

Materials
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