Cobalt and cobalt compounds can occur in the wastewater during “metal-processing and metal-working processes, including anodic oxidation plants and the manufacture of circuit boards”1
Cobalt salts have a carcinogenic effect when absorbed by inhalation, and in the event of oral uptake have a health-hazardous effect after repeated exposure2.
As a measure to prevent any impact on the environment and humans, the limit for the discharge of wastewater into water bodies imposed by Annex 40 of the German wastewater directive is set at 1 mg/l Co1.
The concentration of cobalt in water bodies and wastewater can be measured using the Spectroquant® Cobalt Cell Test Cat. No. 117244. This test offers a swift and precise alternative to conventional spectrometric methods such as ICP-OES (ISO 11885:2009, DIN 38406-24:1993-03, US EPA 200.7) and ICP-MS (US EPA 200.8).3-6
This Application Note describes the determination of cobalt in wastewater metal-processing and metal-working processes using the Spectroquant® Cobalt Cell Test (Cat. No. 117244).
With the Spectroquant® Cobalt Cell Test the cobalt concentration in wastewater can be determined quickly and easily.
The methods are preprogrammed on the corresponding Spectroquant® photometers. Calibration is not necessary. All reagents required for the measurement are included in the test kit.
25 determinations can be carried out per package.
Cobalt(II) ions react with Nitroso-R salt to form an orange-red complex that is determined photometrically.
0.05 – 2.00 mg/l Co
Wastewater from “metal-processing and metal-working processes, including anodic oxidation plants and the manufacture of circuit boards.
Only necessary, if:
Sample needs to be preserved:
pH adjustment is needed:
Sample blank is needed:
Total cobalt should be determined:
For analytical quality assurance:
For the cobalt measurement one of the following Spectroquant® photometers is necessary:
If total cobalt should be determined, one of the following thermoreactors is necessary:
Software for data maintenance
The Spectroquant® Prove Connect to LIMS software package provides an easy way to transfer your data into a preexisting LIMS system. This software can be purchased under:
MQuant® StripScan App
The MQuant® StripScan App is an analytical detection system for the readout of test strips. The app can be downloaded via the IOS app store (the app is currently available for iPhones only). In addition to the app a reference card is necessary which can be purchased under the above-mentioned Cat. No..
Pipettes for pipetting volumes of 0.50 and 5.0 mL
Only necessary for turbid solutions:
Preparing the measurement solutions1)
Sample blank (optional)
If the sample is colored, perform a sample blank before the measurement of the sample itself. This step is necessary to compensate for the intrinsic color of the wastewater.
The sample blank is prepared as followed:
Data transfer Prove spectrophotometers
After measurement transfer the values measured on the Prove spectrophotometer using Prove Connect to LIMS.
1) Due to the instrument design of the Move 100 there may be differences in preparing the reagents as well as performing the measurement. Please see the Move 100 Manual for details.
2) For Details on how to perform the measurement step by step, see the instrument-specific manual
Influences of foreign substances
The cobalt determination is interfered with by in the presence of certain substances. For details see the packaging insert of the test kit.
The Spectroquant® Cobalt Cell Test is a fast and cost-effective alternative to spectrometric methods such as ICP-OES and ICP-MS for the determination of cobalt in wastewater.
The method is preprogrammed on the corresponding Spectroquant® photometers, so there is no need for a time-consuming calibration.
Stringent controls of the raw materials, effective in-process controls and documented final controls are a confirmation for the consistently high quality. Every product batch has an individual certificate, so there is no need to consider checking the quality of the test kit yourself. In addition, the quality certificates confirm consistent batch-to-batch quality.