HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 15:85)
CONDITIONS
column
Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase
[A] water; [B] acetonitrile; (15:85, A:B)
flow rate
1.0 mL/min
column temp.
35 °C
detector
ELSD, 55 °C, 3.5 bar nitrogen
injection
10 μL
Description
Analysis Note
This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany