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Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging.

Nature methods (2017-09-05)
Hsien-Wei Yeh, Omran Karmach, Ao Ji, David Carter, Manuela M Martins-Green, Hui-Wang Ai
ABSTRACT

Red-shifted bioluminescence reporters are desirable for biological imaging. We describe the development of red-shifted luciferins based on synthetic coelenterazine analogs and corresponding mutants of NanoLuc that enable bright bioluminescence. One pair in particular showed superior in vitro and in vivo sensitivity over commonly used bioluminescence reporters. We adapted this pair to develop a bioluminescence resonance-energy-based Antares reporter called Antares2, which offers improved signal from deep tissues.

MATERIALS
Product Number
Brand
Product Description

Supelco
Dextran, analytical standard, for GPC, Set Mp 1,000-400,000
Sigma-Aldrich
Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat, affinity isolated antibody