Merck

Characteristics and chemical compositions of propolis from Ethiopia.

SpringerPlus (2014-06-14)
Ahmed I Rushdi, Nuru Adgaba, Noofal I M Bayaqoob, Ahmed Al-Khazim, Bernd I T Simoneit, Aarif H El-Mubarak, Khalid F Al-Mutlaq
ABSTRACT

Propolis is a sticky material mixed by honeybees to utilize it in protecting their hives from infection by bacteria and fungi. The therapeutic properties of propolis are due to its chemical composition with bio-active compounds; therefore, researchers are interested in studying its chemical constituents and biological properties. The main objective of this study is to determine the chemical compositions, characteristics and relative concentrations of organic compounds in the extractable organic matter of propolis samples collected from four different areas in Ethiopia. The propolis samples were extracted with a mixture of dichloromethane and methanol and analyzed by gas chromatography-mass spectrometry (GC-MS).The results showed that the total extract yields ranged from 27.2% to 64.2% (46.7 ± 19.1%). The major compounds were triterpenoids (85.5 ± 15.0% of the total extracts, mainly α-, β-amyrins and amyryl acetates), n-alkanes (5.8 ± 7.5%), n-alkenes (6.2 ± 7.0%,), methyl n-alkanoates (0.4 ± 0.2%), and long chain wax esters (0.3 to 2.1%). The chemical compositions of these propolis samples indicate that they are potential sources of natural bio-active compounds for biological and pharmacological applications.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
N,O-Bis(trimethylsilyl)trifluoroacetamide, ≥99%
Supelco
N,O-Bis(trimethylsilyl)trifluoroacetamide with trimethylchlorosilane, with 1% trimethylchlorosilane, for GC derivatization, LiChropur