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Simultaneous quantification of curdione, furanodiene and germacrone in rabbit plasma using liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study.

Biomedical chromatography : BMC (2015-02-14)
Baolian Wang, Shuang Yang, Li Sheng, Yan Li
ABSTRACT

A simple, rapid and sensitive method was developed for the simultaneous quantification of curdione, furanodiene and germacrone in rabbit plasma using a LC-MS/MS analysis. The plasma sample preparation was a simple deproteinization by the addition of 3 vols of acetonitrile followed by centrifugation. The analytes and internal standard (IS) costunolide were separated on a Zorbax SB-C18 column (3.5 µm, 2.1 × 100 mm) with mobile phase of methanol-water (90:10, v/v) containing 0.1% formic acid at a flow rate of 0.3 mL/min with an operating temperature of 25°C. Detection was carried out by atmospheric pressure chemical ionization in positive ion selected reaction monitoring mode. Linear detection responses were obtained for the three test compounds ranging from 5 to 5000 ng/mL and the lower limits of quantitation were 5-10 ng/mL. The intra- and inter-day precisions (relative standard deviations) were within 9.4% for all analytes, while the deviation of assay accuracies was within ±10.0%. The average recoveries of analytes were >80.0%. All analytes were proved to be stable during all sample storage, preparation and analytical procedures. The method was successfully applied to the pharmacokinetic study of the three compounds after vaginal drug delivery of Baofukang suppository to rabbit.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methanol, SAJ special grade
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Methanol, suitable for HPLC
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Methanol, anhydrous, 99.8%
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Methanol, JIS 300, ≥99.8%, for residue analysis
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Methanol, ≥99.8%, for chromatography
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Methanol, ≥99.8%, suitable for absorption spectrum analysis
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Methanol, SAJ first grade, ≥99.5%
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Methanol, JIS 1000, ≥99.8%, for residue analysis
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Methanol, JIS special grade, ≥99.8%
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Acetonitrile solution, contains 0.05 % (v/v) trifluoroacetic acid
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Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
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Acetonitrile solution, contains 0.1 % (v/v) formic acid, suitable for HPLC
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Acetonitrile solution, contains 0.05 % (w/v) ammonium formate, 0.1 % (v/v) formic acid, 5 % (v/v) water, suitable for HPLC
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Methanol, for residue analysis, JIS 5000
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Methanol, HPLC Plus, ≥99.9%, poly-coated bottles
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Acetonitrile, ≥99.8%, for residue analysis, JIS 1000
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Acetonitrile, ≥99.8%, suitable for HPLC
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Acetonitrile, SAJ first grade, ≥99.0%
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Acetonitrile, JIS special grade, ≥99.5%
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Acetonitrile, ≥99.8%, for residue analysis, JIS 300
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Acetonitrile, for residue analysis, JIS 5000
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Acetonitrile, for chromatography
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Acetonitrile, ≥99.5%, ACS reagent
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Acetonitrile, anhydrous, 99.8%
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Methanol-12C, 99.95 atom % 12C
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Acetonitrile, electronic grade, 99.999% trace metals basis
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Costunolide, ≥97% (HPLC)
Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%
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Acetonitrile