Merck

Bioactive products formed in humans from fish oils.

Journal of lipid research (2015-07-17)
Carsten Skarke, Naji Alamuddin, John A Lawson, Xuanwen Li, Jane F Ferguson, Muredach P Reilly, Garret A FitzGerald
ABSTRACT

Resolvins, maresins, and protectins can be formed from fish oils. These specialized pro-resolving mediators (SPMs) have been implicated in the resolution of inflammation. Synthetic versions of such SPMs exert anti-inflammatory effects in vitro and when administered to animal models. However, their importance as endogenous products formed in sufficient amounts to exert anti-inflammatory actions in vivo remains speculative. We biased our ability to detect SPMs formed in healthy volunteers by supplementing fish oil in doses shown previously to influence blood pressure and platelet aggregation under placebo-controlled conditions. Additionally, we sought to determine the relative formation of SPMs during an acute inflammatory response and its resolution, evoked in healthy volunteers by bacterial lipopolysaccharide (LPS). Bioactive lipids, enzymatic epoxyeicosatrienoic acids (EETs), and free radical-catalyzed prostanoids [isoprostanes (iPs)] formed from arachidonic acid and the fish oils, served as comparators. Despite the clear shift from ω-6 to ω-3 EETs and iPs, we failed to detect a consistent signal, in most cases, of SPM formation in urine or plasma in response to fish oil, and in all cases in response to LPS on a background of fish oil. Our results question the relevance of these SPMs to the putative anti-inflammatory effects of fish oils in humans.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%, poly-coated bottles
Sigma-Aldrich
Methanol, for residue analysis, JIS 5000
Sigma-Aldrich
Methanol, SAJ special grade
Sigma-Aldrich
Methanol, suitable for HPLC
Sigma-Aldrich
Methanol, NMR reference standard
Sigma-Aldrich
Methanol, JIS 1000, ≥99.8%, for residue analysis
Sigma-Aldrich
Methanol, ≥99.8%, suitable for absorption spectrum analysis
Sigma-Aldrich
Methanol, ≥99.8%, for chromatography
Sigma-Aldrich
Methanol, JIS special grade, ≥99.8%
Sigma-Aldrich
Methanol, SAJ first grade, ≥99.5%
Sigma-Aldrich
Methanol, JIS 300, ≥99.8%, for residue analysis
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Sigma-Aldrich
Dehydro-L-(+)-ascorbic acid dimer, ≥80% (enzymatic)
Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
Sigma-Aldrich
(L)-Dehydroascorbic acid
Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%