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Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood.

Prostaglandins, leukotrienes, and essential fatty acids (2015-03-11)
M Nurhasan, N Roos, J J Aristizabal Henao, C Chamnan, K D Stark, L Lauritzen
ABSTRACT

Fatty acid analysis requires standardized collection and storage of samples, which can be a challenge under field conditions. This study describes the effect of storage temperature on fatty acid composition in two sets of whole blood samples collected from 66 children in a rural area in Cambodia. The samples were stored with butylated hydroxytoluene at -20 °C and -80 °C and the latter required extra transfers due to storage facility limitation. Fatty acid composition was analyzed by high-throughput gas-chromatography and evaluated by paired t-tests and Bland-Altman plots. Total amounts of fat in -20 °C and -80 °C samples did not differ, but there was relatively more highly unsaturated fatty acids (15.8 ± 2.7 vs. 14.4 ± 2.5%, p < 0.001) and a lower n-6/n-3 ratio (6.4 ± 1.4 vs. 6.9 ± 1.4, p < 0.001) in the -20 °C samples. Our results indicate that the importance of storage temperature should be evaluated in the context of storage facility availability and risk of temperature fluctuations during transport.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Nitroterephthalic acid, ≥99%
Sigma-Aldrich
L-Lysine monohydrochloride, SAJ special grade, ≥99.0%
Sigma-Aldrich
L-Lysine monohydrochloride, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
L-Lysine monohydrochloride, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
L-Lysine monohydrochloride, BioUltra, ≥99.5% (AT)