Merck
  • Home
  • Search Results
  • Molecular and functional characterization of porcine Siglec-3/CD33 and analysis of its expression in blood and tissues.

Molecular and functional characterization of porcine Siglec-3/CD33 and analysis of its expression in blood and tissues.

Developmental and comparative immunology (2015-04-22)
B Álvarez, Z Escalona, H Uenishi, D Toki, C Revilla, M Yuste, M Gómez Del Moral, F Alonso, A Ezquerra, J Domínguez
ABSTRACT

A cDNA clone encoding a 380 a-a type 1 transmembrane protein with homology to human Siglec-3/CD33 was obtained from a swine small intestine library. An analysis of protein sequence identified two immunoglobulin-like domains, a transmembrane region, and a carboxi-terminal tail with two tyrosine-based signalling motifs. Binding assays of Siglec-3 transfected CHO cells to polyacrylamide glycoconjugates showed a preference for α2-6-linked sialic acids. Using mAbs raised against a fragment containing the two Ig-like domains, porcine Siglec-3 was found to be expressed on monocytes and granulocytes, and their bone marrow precursors. It was also detected in lymph node, splenic and alveolar macrophages. MAbs immunoprecipitated, from granulocyte lysates, a protein of 51-60 kDa under both non-reducing and reducing conditions. MAbs were also used to analyse functional activity of Siglec-3 on bone marrow and blood cells. Engagement of Siglec-3 by mAb had no apparent effect on cell proliferation or cytokine production.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-Lysine monohydrochloride, SAJ special grade, ≥99.0%
Sigma-Aldrich
L-Lysine monohydrochloride, BioUltra, ≥99.5% (AT)
Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%
Sigma-Aldrich
L-Lysine monohydrochloride, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
L-Lysine monohydrochloride, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
Sigma-Aldrich
Imiquimod, ≥98% (HPLC), solid
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Sigma-Aldrich
Methanol, spectrophotometric grade, ≥99%
Sigma-Aldrich
Methanol, NMR reference standard
Sigma-Aldrich
Methanol, suitable for HPLC
Sigma-Aldrich
Methanol, SAJ first grade, ≥99.5%
Sigma-Aldrich
Methanol, ≥99.8%, for chromatography
Sigma-Aldrich
Methanol, JIS special grade, ≥99.8%
Sigma-Aldrich
Methanol, SAJ special grade
Sigma-Aldrich
Methanol, JIS 1000, ≥99.8%, for residue analysis
Sigma-Aldrich
Methanol, ≥99.8%, suitable for absorption spectrum analysis
Sigma-Aldrich
Methanol, JIS 300, ≥99.8%, for residue analysis
Sigma-Aldrich
Methanol, anhydrous, ≥99.5%
Sigma-Aldrich
Methanol, AR, ≥99.5%
Sigma-Aldrich
Methanol, LR, ≥99%
Sigma-Aldrich
Methanol, for residue analysis, JIS 5000
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%, poly-coated bottles