Skip to Content
Merck
  • Flavonoid composition and antioxidant activities of Chinese local pummelo (Citrus grandis Osbeck.) varieties.

Flavonoid composition and antioxidant activities of Chinese local pummelo (Citrus grandis Osbeck.) varieties.

Food chemistry (2014-05-20)
Wanpeng Xi, Bo Fang, Qiyang Zhao, Bining Jiao, Zhiqin Zhou
ABSTRACT

China is one of the most important diversity centres of the genus Citrus L. and is particularly rich in pummelo germplasm. In this study, the flavonoids in the peels and pulps of 28 Chinese local pummelos and four grapefruits were determined by optimised Ultra Performance Liquid Chromatography (UPLC), and their antioxidant capacities were evaluated using 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH), ferric reducing/antioxidant power (FRAP) and superoxide anion methods. We found that naringin was the predominant flavonoid in pummelo, while naringin and neohesperidin were the predominant flavonoids in grapefruit. The fruit peels of Citrus paradisi cvs. Rio Red and Cocktail had the highest contents of naringin (9871.69mg/kg FW) and neohesperidin (7011.15mg/kg FW), respectively. Overall, C. paradisi cvs. Cocktail, Rio Red and Changshanhuyou, Citrus grandis cvs. 28-19, Chandler, and Hongxinyou contained more flavonoids and exhibited higher antioxidant capacities and are potentially good sources of phytochemicals and natural antioxidants.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Dimethyl sulfoxide, suitable for HPLC
Sigma-Aldrich
Acetonitrile, for chromatography
Sigma-Aldrich
Acetonitrile, for residue analysis, JIS 5000
Sigma-Aldrich
Dimethyl sulfoxide, SAJ first grade, ≥99.0%
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.0%, suitable for absorption spectrum analysis
Sigma-Aldrich
Acetonitrile, ≥99.8%, for residue analysis, JIS 300
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5%
Sigma-Aldrich
Acetonitrile, ≥99.8%, suitable for HPLC
Millipore
Bifido Selective Supplement B, suitable for microbiology
Supelco
Hesperidin, analytical standard
Sigma-Aldrich
Acetonitrile, SAJ first grade, ≥99.0%
Sigma-Aldrich
Dimethyl sulfoxide solution, 50 wt. % in H2O
Sigma-Aldrich
Acetonitrile, ≥99.8%, for residue analysis, JIS 1000
Sigma-Aldrich
Dimethyl sulfoxide, JIS special grade, ≥99.0%
Sigma-Aldrich
Acetonitrile, JIS special grade, ≥99.5%
Sigma-Aldrich
Hesperetin, ≥95%
Supelco
Acetonitrile, analytical standard
Sigma-Aldrich
Acetonitrile, ReagentPlus®, 99%
Sigma-Aldrich
Dimethyl sulfoxide, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Dimethyl sulfoxide, ACS reagent, ≥99.9%
Sigma-Aldrich
Acetonitrile, ACS reagent, ≥99.5%
Naringin, European Pharmacopoeia (EP) Reference Standard
USP
Glacial acetic acid, United States Pharmacopeia (USP) Reference Standard
Hesperidin, primary reference standard
Supelco
Dimethyl sulfoxide, for inorganic trace analysis, ≥99.99995% (metals basis)
Supelco
Naringin, analytical standard
USP
Dimethyl sulfoxide, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Acetonitrile, electronic grade, 99.999% trace metals basis
Hesperidin, European Pharmacopoeia (EP) Reference Standard
USP
Residual Solvent Class 2 - Acetonitrile, United States Pharmacopeia (USP) Reference Standard