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Synthesis and LC-MS/MS analysis of desmosine-CH2, a potential internal standard for the degraded elastin biomarker desmosine.

Organic & biomolecular chemistry (2014-10-31)
Yuko Murakami, Rina Suzuki, Hiroto Yanuma, Jiangtao He, Shuren Ma, Gerard M Turino, Yong Y Lin, Toyonobu Usuki
ABSTRACT

Desmosine-CH2, an analog of the elastic tissue degradation biomarker desmosine, can be regarded as a potential internal standard for precise quantification of desmosines by LC-MS/MS. In this study, the chemical synthesis of desmosine-CH2 was completed in 22% overall yield in five steps. The LC-MS/MS analysis of desmosine-CH2 was also achieved.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Heptafluorobutyric acid, 98%
Sigma-Aldrich
Heptafluorobutyric acid, ≥99.0% (GC)
Supelco
Heptafluorobutyric acid, suitable for ion chromatography, ≥99.5% (GC)
Supelco
Heptafluorobutyric acid solution, 0.5 M in H2O, LiChropur, suitable for ion pair chromatography