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Kinetics of sucrose conversion to fructo-oligosaccharides using enzyme (invertase) under free condition.

Bioprocess and biosystems engineering (2014-06-19)
Deepa C Khandekar, Tapas Palai, Aman Agarwal, Prashant K Bhattacharya
ABSTRACT

The study reports the synthesis of fructo-oligosaccharide (FOS) from sucrose using invertase derived from Saccharomyces cerevisiae. The reaction was conducted in a batch mode under free enzyme condition. Fructo-oligosaccharide formation was detected at a high sucrose concentration of over 200 g/L. The investigation was extended to study the effect of different parameters such as initial sucrose concentration (ISC), pH, and enzyme concentration. A maximum FOS yield of 10 % (dry basis) was observed using 525 g/L of ISC, with 6 U/mL of the enzyme, and pH 5.5 at 40 °C. 1-Kestose was the major product of among different forms of FOS. The FOS yield increased with an increase in sucrose concentration up to 525 g/L, beyond which it started to decrease. However, the maximum FOS yield was not affected by the increasing concentration of the enzyme beyond a certain level (2 U/mL). Furthermore, the activity of enzyme slightly increased with an increase in the pH up to 6, and thereafter it declined. Addition of glucose decreased the FOS yield because of enzyme inhibition. A five-step, ten-parameter model was developed, for which the simulation was performed in COPASI. The results predicted by the model were consistent with the experimental data.

MATERIALS
Product Number
Brand
Product Description

Supelco
1-Kestose, analytical standard
Sigma-Aldrich
4-Hydroxybenzhydrazide, ≥97%
Supelco
Nystose, analytical standard