• Home
  • Search Results
  • Fresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compounds.

Fresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compounds.

Artificial cells, nanomedicine, and biotechnology (2013-02-01)
Hakki Mevlut Ozcan, Ayten Sagiroglu
ABSTRACT

In this study, a novel fresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compounds was developed. The biosensor was constructed by immobilizing tissue homogenate of fresh broad (Vicia faba) on to glassy carbon electrode. For the stability of the biosensor, general immobilization techniques were used to secure the fresh broad tissue homogenate in gelatin-glutaraldehyde cross-linking matrix. In the optimization and characterization studies, the amount of fresh broad tissue homogenate and gelatin, glutaraldehyde percentage, optimum pH, optimum temperature and optimum buffer concentration, thermal stability, interference effects, linear range, storage stability, repeatability and sample applications (Wine, beer, fruit juices) were also investigated. Besides, the detection ranges of thirteen phenolic compounds were obtained with the help of the calibration graphs. A typical calibration curve for the sensor revealed a linear range of 5-60 μM catechol. In reproducibility studies, variation coefficient (CV) and standard deviation (SD) were calculated as 1.59%, 0.64×10(-3) μM, respectively.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
p-Cresol, puriss. p.a., ≥99.0% (GC)
Supelco
Quercetin, Pharmaceutical Secondary Standard; Certified Reference Material
Levodopa, European Pharmacopoeia (EP) Reference Standard
USP
Resorcinol, United States Pharmacopeia (USP) Reference Standard
Glycine, European Pharmacopoeia (EP) Reference Standard
Supelco
L-Ascorbic acid, certified reference material, TraceCERT®
Hymecromone impurity A, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Sodium sulfite, puriss., meets analytical specification of Ph. Eur., BP, NF, anhydrous, 95.0-100.0% (iodometric)
Sigma-Aldrich
p-Cresol, 99%
Sigma-Aldrich
Sodium sulfite, ACS reagent, ≥98.0%
Sigma-Aldrich
Sodium sulfite, ≥98%
Sigma-Aldrich
Sodium sulfite, puriss. p.a., reag. Ph. Eur., anhydrous, 98-100.0% (iodometric)
Resorcinol, European Pharmacopoeia (EP) Reference Standard
USP
Quercetin, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Sodium sulfite, anhydrous, Redi-Dri, reagent grade, ≥98%
USP
Glycine, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Sodium hydrosulfide hydrate
USP
Levodopa, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
L-Ascorbic acid, powder, suitable for cell culture, γ-irradiated
Sigma-Aldrich
L-Ascorbic acid, suitable for plant cell culture
Sigma-Aldrich
L-Ascorbic acid, BioXtra, ≥99.0%, crystalline
Sigma-Aldrich
L-Ascorbic acid, reagent grade, crystalline
Sigma-Aldrich
L-Ascorbic acid, suitable for cell culture, suitable for plant cell culture, ≥98%
Sigma-Aldrich
L-Ascorbic acid, reagent grade
Sigma-Aldrich
Thiourea, ACS reagent, ≥99.0%
Sigma-Aldrich
L-Ascorbic acid, meets USP testing specifications
Sigma-Aldrich
Thiourea, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Resorcinol, BioXtra, ≥99%
Sigma-Aldrich
Rutin hydrate, ≥94% (HPLC), powder
Sigma-Aldrich
Glycine, BioXtra, ≥99% (titration)