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Fresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compounds.

Artificial cells, nanomedicine, and biotechnology (2013-02-01)
Hakki Mevlut Ozcan, Ayten Sagiroglu
ABSTRACT

In this study, a novel fresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compounds was developed. The biosensor was constructed by immobilizing tissue homogenate of fresh broad (Vicia faba) on to glassy carbon electrode. For the stability of the biosensor, general immobilization techniques were used to secure the fresh broad tissue homogenate in gelatin-glutaraldehyde cross-linking matrix. In the optimization and characterization studies, the amount of fresh broad tissue homogenate and gelatin, glutaraldehyde percentage, optimum pH, optimum temperature and optimum buffer concentration, thermal stability, interference effects, linear range, storage stability, repeatability and sample applications (Wine, beer, fruit juices) were also investigated. Besides, the detection ranges of thirteen phenolic compounds were obtained with the help of the calibration graphs. A typical calibration curve for the sensor revealed a linear range of 5-60 μM catechol. In reproducibility studies, variation coefficient (CV) and standard deviation (SD) were calculated as 1.59%, 0.64×10(-3) μM, respectively.

MATERIALS
Product Number
Brand
Product Description

SAFC
Glycine
Sigma-Aldrich
Glycine, BioUltra, for molecular biology, ≥99.0% (NT)
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L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, Ph. Eur., 99.7-100.5% (oxidimetric)
Supelco
p-Cresol, analytical standard
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Sodium sulfite, BioUltra, anhydrous, ≥98% (RT)
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Glycine, BioXtra, ≥99% (titration)
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p-Cresol, ≥99%, FG
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L-Ascorbic acid, 99%
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Glycine, puriss. p.a., reag. Ph. Eur., buffer substance, 99.7-101% (calc. to the dried substance)
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Glycine, ACS reagent, ≥98.5%
Supelco
Mettler-Toledo Calibration substance ME 18555, Benzoic acid, analytical standard, for the calibration of the thermosystem 900, traceable to primary standards (LGC)
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L-Ascorbic acid, puriss. p.a., ≥99.0% (RT)
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Glycine, meets analytical specification of Ph. Eur., BP, USP, 99-101% (based on anhydrous substance)
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Glutaraldehyde solution, technical, ~50% in H2O (5.6 M)
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Glycine, tested according to Ph. Eur.
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L-Ascorbic acid, tested according to Ph. Eur.
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L-Ascorbic acid, BioUltra, ≥99.5% (RT)
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Resorcinol, meets analytical specification of Ph. Eur., BP, 98.5-100.5% (calc. to the dried substance)
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L-Ascorbic acid, suitable for cell culture, suitable for plant cell culture, ≥98%
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L-Ascorbic acid, meets USP testing specifications
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Thiourea, ACS reagent, ≥99.0%
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L-Ascorbic acid, powder, suitable for cell culture, γ-irradiated
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Thiourea, ReagentPlus®, ≥99.0%
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L-Ascorbic acid, BioXtra, ≥99.0%, crystalline
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L-Ascorbic acid, suitable for plant cell culture
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L-Ascorbic acid, reagent grade, crystalline
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L-Ascorbic acid, reagent grade
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Resorcinol, BioXtra, ≥99%
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Rutin hydrate, ≥94% (HPLC), powder
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Glycine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, ≥98.5%