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  • [Salidroside biosynthesis pathway: the initial reaction and glycosylation of tyrosol].

[Salidroside biosynthesis pathway: the initial reaction and glycosylation of tyrosol].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology (2012-06-21)
Lanqing Ma, Chunmei Liu, Hansong Yu, Jixing Zhang, Dongyao Gao, Yanfang Li, Younian Wang
ABSTRACT

Salidroside, the 8-O-beta-D-glucoside of tyrosol, is a novel adaptogenic drug extracted from the medicinal plant Rhodiola sachalinensis A. Bor. Due to the scarcity of R. sachalinensis and its low yield of salidroside, there is great interest in enhancing the production of salidroside by biotechnological process. Glucosylation of tyrosol is thought to be the final step in salidroside biosynthesis. In our related works, three UGT clones were isolated from the roots and the cultured cells. Our intention was to combine the catalytic specificity of these UGTs in vitro in order to change the level of salidroside in vivo by over-expression of the above UGTs. However, as the aglycone substrate of salidroside, the biosynthetic pathway of tyrosol and its regulation are less well understood. The results of related studies revealed that there are two different possibilities for the tyrosol biosynthetic pathway. One possibility is that tyrosol is produced from a p-coumaric acid precursor, which is derived mainly from phenylalanine. The second possibility is that the precursor of tyrosol might be tyramine, which is synthesized from tyrosine. Our previous work demonstrated that over-expression of the endogenous phenylalanine ammonia-lyase gene (PALrs1) and accumulation of p-coumaric acid did not facilitate tyrosol biosynthesis. In contrast, the data presented in our recent work provide in vitro and in vivo evidence that the tyrosine decarboxylase (RsTyrDC) is most likely to have an important function in the initial reaction of the salidroside biosynthesis pathway in R. Sachalinensis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Salidroside, ≥95% (LC/MS-ELSD)
Supelco
Salidroside, analytical standard