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  • Investigation of malic acid production in Aspergillus oryzae under nitrogen starvation conditions.

Investigation of malic acid production in Aspergillus oryzae under nitrogen starvation conditions.

Applied and environmental microbiology (2013-07-31)
Christoph Knuf, Intawat Nookaew, Stephen H Brown, Michael McCulloch, Alan Berry, Jens Nielsen
ABSTRACT

Malic acid has great potential for replacing petrochemical building blocks in the future. For this application, high yields, rates, and titers are essential in order to sustain a viable biotechnological production process. Natural high-capacity malic acid producers like the malic acid producer Aspergillus flavus have so far been disqualified because of special growth requirements or the production of mycotoxins. As A. oryzae is a very close relative or even an ecotype of A. flavus, it is likely that its high malic acid production capabilities with a generally regarded as safe (GRAS) status may be combined with already existing large-scale fermentation experience. In order to verify the malic acid production potential, two wild-type strains, NRRL3485 and NRRL3488, were compared in shake flasks. As NRRL3488 showed a volumetric production rate twice as high as that of NRRL3485, this strain was selected for further investigation of the influence of two different nitrogen sources on malic acid secretion. The cultivation in lab-scale fermentors resulted in a higher final titer, 30.27 ± 1.05 g liter(-1), using peptone than the one of 22.27 ± 0.46 g liter(-1) obtained when ammonium was used. Through transcriptome analysis, a binding site similar to the one of the Saccharomyces cerevisiae yeast transcription factor Msn2/4 was identified in the upstream regions of glycolytic genes and the cytosolic malic acid production pathway from pyruvate via oxaloacetate to malate, which suggests that malic acid production is a stress response. Furthermore, the pyruvate carboxylase reaction was identified as a target for metabolic engineering, after it was confirmed to be transcriptionally regulated through the correlation of intracellular fluxes and transcriptional changes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-(−)-Malic acid disodium salt, ≥95% (titration)
Sigma-Aldrich
DL-Malic acid, ≥98% (capillary GC)
Sigma-Aldrich
Malic acid, meets USP/NF testing specifications
Sigma-Aldrich
DL-Malic acid, SAJ first grade, ≥99.0%
Sigma-Aldrich
D-(+)-Malic acid, unnatural form, ≥97.0% (T)
Sigma-Aldrich
DL-Malic acid, ReagentPlus®, ≥99%
Sigma-Aldrich
DL-Malic acid, 99%
Supelco
D-Malic acid, analytical standard