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Multimodal contrast agents for in vivo neuroanatomical analysis of monosynaptic connections.

Biomaterials (2013-06-25)
Giuseppe Gambino, Jörn Engelmann, Lorenzo Tei, Mauro Botta, Nikos K Logothetis, Ilgar Mamedov
ABSTRACT

We developed and examined the applicability of two multimodal paramagnetic contrast agents for the longitudinal in vivo investigations of the brain projections. The classical dextran based neuroanatomical tracer was conjugated with mono- and bimetal Gd(3+) complexes and an optical reporter. Relaxometric studies of both tracer molecules were performed in vitro followed by in cellulo MR and microscopy investigations. Finally, tracers were injected into the motor cortex of the rat brain; uptake and transporting properties were compared by MRI. The advantage of the multimodal approach was taken and histological studies were performed on the same animals. The histology results confirm the MRI studies demonstrating that the applied tracers labelled anterogradely the regions known for their connections with the motor cortex of the rat brain.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 48,000-90,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 9,000-11,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 150,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 15,000-30,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 1,500,000-2,800,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 35,000-45,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~40,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~2,000,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~6,000
Supelco
Dextran, analytical standard, for GPC, Set Mp 1,000-400,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~70,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr 450,000-650,000
Sigma-Aldrich
Dextran, enzymatic synth.
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 670,000
Supelco
Dextran from Leuconostoc mesenteroides, for GPC, analytical standard, Mw 50,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 410,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 12,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 1,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 25,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 5,000
Sigma-Aldrich
Dextran solution from Leuconostoc mesenteroides, 20 % (w/w) (Autoclaved)
Supelco
Dextran, analytical standard, for GPC, 270,000
Supelco
Dextran, analytical standard, for GPC, 670,000
Supelco
Dextran, analytical standard, for GPC, 150,000
Supelco
Dextran, analytical standard, for GPC, 80,000
Supelco
Dextran, analytical standard, for GPC, 410,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr 15,000-25,000
Supelco
Dextran, analytical standard, for GPC, 5,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, Mr ~60,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, Mr ~200,000