Merck

The human cerebrospinal fluid metabolome.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2008-05-27)
David S Wishart, Michael J Lewis, Joshua A Morrissey, Mitchel D Flegel, Kevin Jeroncic, Yeping Xiong, Dean Cheng, Roman Eisner, Bijaya Gautam, Dan Tzur, Summit Sawhney, Fiona Bamforth, Russ Greiner, Liang Li
ABSTRACT

With continuing improvements in analytical technology and an increased interest in comprehensive metabolic profiling of biofluids and tissues, there is a growing need to develop comprehensive reference resources for certain clinically important biofluids, such as blood, urine and cerebrospinal fluid (CSF). As part of our effort to systematically characterize the human metabolome we have chosen to characterize CSF as the first biofluid to be intensively scrutinized. In doing so, we combined comprehensive NMR, gas chromatography-mass spectrometry (GC-MS) and liquid chromatography (LC) Fourier transform-mass spectrometry (FTMS) methods with computer-aided literature mining to identify and quantify essentially all of the metabolites that can be commonly detected (with today's technology) in the human CSF metabolome. Tables containing the compounds, concentrations, spectra, protocols and links to disease associations that we have found for the human CSF metabolome are freely available at http://www.csfmetabolome.ca.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
α-Ketoglutaric acid, ≥98.5% (NaOH, titration)
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Methanol, SAJ special grade
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Methanol, suitable for HPLC
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Acetone, for residue analysis, JIS 5000
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Acetone, suitable for HPLC
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Acetone, for chromatography, ≥99.8%
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L-Alanine, SAJ special grade, ≥99.0%
Supelco
L-Aspartic acid, Pharmaceutical Secondary Standard; Certified Reference Material
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Glycerin, Pharmaceutical Secondary Standard; Certified Reference Material
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Propylene glycol, Pharmaceutical Secondary Standard; Certified Reference Material
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L-Alanine, Pharmaceutical Secondary Standard; Certified Reference Material
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L-Tyrosine, FG
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Acetic acid, natural, ≥99.5%, FG
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Methanol, anhydrous, 99.8%
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3-Hydroxybutyric acid, 95%
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Acetic acid, ≥99.5%, FCC, FG
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L-Leucine, 99%, FG
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L-Alanine, ≥99.0% (NT)
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Glycerol solution, 83.5-89.5% (T)
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Acetone, suitable for HPLC, ≥99.9%
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Urea-12C, 99.9 atom % 12C
SAFC
L-Aspartic acid
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L-Threonine
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L-Valine
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L-Tyrosine
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Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)