Merck
  • Home
  • Search Results
  • Effect of hydration state and storage media on corneal biomechanical response from in vitro inflation tests.

Effect of hydration state and storage media on corneal biomechanical response from in vitro inflation tests.

Journal of refractive surgery (Thorofare, N.J. : 1995) (2013-07-04)
Sabine Kling, Susana Marcos
ABSTRACT

To evaluate corneal deformation with varying intraocular pressure and the dependency of the biomechanical response on the corneal hydration state, modulated by the storage solutions or postmortem period. Thirty fresh enucleated porcine eyes were used for in vitro whole eye globe inflation experiments. The eyes were separated into five groups and treated with different solutions: 20% dextran, 8% dextran, 0.125% riboflavin-20% dextran, Optisol-GS (Bausch & Lomb, Rochester, NY), and one control group of virgin (untreated) eyes. Intraocular pressure was increased (from 15 to 55 mm Hg) and decreased (to 15 mm Hg) in 5-mm Hg steps and Scheimpflug images were taken at each step. Measurements were repeated after 24 hours. Thickness and curvature changes were analyzed as a function of intraocular pressure. Corneal deformation differed across conditions and hydration states. Dehydration by any dextran solution increased the hysteresis after the inflation/deflation cycle (14.29 vs 22.07 to 41.75 μm), whereas overnight hydration did not lead to a significant difference. Compared to control corneas, corneas treated with Optisol-GS showed the most similar behavior. Corneas treated with 0.125% riboflavin-20% dextran deformed most (Δthickness(max) = 38.27 μm), indicating a softening of the corneal tissue compared to control corneas (23.18 μm) and corneas treated with 8% dextran (21.01 μm) and 20% dextran (29.07 μm). Dextran instillation decreased corneal thickness on average to 56.5% at 0 hours and 72.7% at 24 hours. Corneal hydration and tissue preservation changed corneal biomechanics, in particular its relaxation over a period of 24 hours.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
(−)-Riboflavin, meets USP testing specifications
Sigma-Aldrich
(−)-Riboflavin, from Eremothecium ashbyii, ≥98%
Sigma-Aldrich
(−)-Riboflavin, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 48,000-90,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 9,000-11,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 150,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 15,000-30,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 1,500,000-2,800,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 35,000-45,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~40,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~2,000,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~6,000
Supelco
Dextran, analytical standard, for GPC, Set Mp 1,000-400,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~70,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr 450,000-650,000
Sigma-Aldrich
Dextran, enzymatic synth.
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 670,000
Supelco
Dextran from Leuconostoc mesenteroides, for GPC, analytical standard, Mw 50,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 410,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 12,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 1,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 25,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 5,000
Sigma-Aldrich
Dextran solution from Leuconostoc mesenteroides, 20 % (w/w) (Autoclaved)
Supelco
Dextran, analytical standard, for GPC, 270,000
Supelco
Dextran, analytical standard, for GPC, 670,000
Supelco
Dextran, analytical standard, for GPC, 150,000
Supelco
Dextran, analytical standard, for GPC, 80,000
Supelco
Dextran, analytical standard, for GPC, 410,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr 15,000-25,000