Merck
  • Home
  • Search Results
  • [The role of thiol antioxidants in restoring mitochondrial functions, modified by microbial metabolites].

[The role of thiol antioxidants in restoring mitochondrial functions, modified by microbial metabolites].

Biofizika (2012-11-10)
N I Fedotcheva, V V Teplova, N V Beloborodova
ABSTRACT

The effects of phenolic acids of microbial origin on mitochondrial functions and the possibility of removing their effects by thiol antioxidants dithiotreitol and N-acethylcysteine were studied. The action of some phenolic acids on the redox state of NADH, the membrane potential and calcium capacity of mitochondria is due to their interaction with thiol groups. The partial restoration of mitochondrial functions occurred in the presence of dithiotreitol and N-acethylcysteine, the full recovery (short-term duration) was promoted by the combined action of dithiotreitol and menadione (vitamin K3). It was found that the protective effect of thiol antioxidants became prooxidant one, if the medium contained free iron and compounds with a quinone structure, capable of entering into a redox cycle with thiols. It is shown that the interaction of thiols with iron and menadione is accompanied by absorption of oxygen to form superoxide anion. Prooxidant effect of thiol antioxidants may explain the absence of the protective effect at the later stages of sepsis and systemic inflammatory syndrome.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Menadione sodium bisulfite, BioReagent, suitable for cell culture, ≥95% (TLC)
Sigma-Aldrich
Menadione sodium bisulfite, ≥95% (TLC)
Supelco
Mettler-Toledo Calibration substance ME 18555, Benzoic acid, analytical standard, for the calibration of the thermosystem 900, traceable to primary standards (LGC)
Sigma-Aldrich
Menadione, crystalline
Sigma-Aldrich
Menadione, meets USP testing specifications
Sigma-Aldrich
Benzoic acid, meets analytical specification of Ph. Eur., BP, USP, FCC, E210, 99.5-100.5% (alkalimetric)
Sigma-Aldrich
Benzoic acid, SAJ first grade, ≥99.5%
Sigma-Aldrich
Benzoic acid, SAJ special grade, ≥99.5%
Supelco
Benzoic acid, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Melting point standard 121-123°C, analytical standard
Sigma-Aldrich
Benzoic acid, purified by sublimation, ≥99%
Sigma-Aldrich
Potassium benzoate, ReagentPlus®, 99%
Sigma-Aldrich
Benzoic acid, natural, ≥99.5%, FCC, FG
Sigma-Aldrich
Benzoic acid, ≥99.5%, FCC, FG
Sigma-Aldrich
Benzoic acid, ReagentPlus®, 99%
Sigma-Aldrich
Benzoic acid, ACS reagent, ≥99.5%
Supelco
Benzoic acid, certified reference material for titrimetry, certified by BAM, ≥99.5%
Sigma-Aldrich
Benzoic acid, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.9% (alkalimetric)
Supelco
Menadione (K3), analytical standard
Menadione, European Pharmacopoeia (EP) Reference Standard
Supelco
SS Benzoic Acid, 2000 μg/mL in dichloromethane, analytical standard
Supelco
Benzoic acid, Standard for quantitative NMR, TraceCERT®