A methods for the simultaneous determination for 1-kestose (GF2) and nystose (GF3) in foods and vegetables by high performance anion exchange chromatography with integrated pulsed amperometric detection (HPAEC-PAD) was developed in this study. With a gradient program, and NaOH and sodium acetate as mobile phase, the two fructo-oligosaccharides (FOS) were separated on a CarboPac PA1 column and then detected by a gold electrode. The linear range were 1.22 - 15.20 microg/g and 1.64 - 18.30 microg/g for GF2 and GF3, and the calculated method detection limit (MDL) were 0.085 microg/g and 0.089 microg/g, respectively. The relative standard deviation of GF2 (6.08 microg/g) and GF3 (7.32 microg/g) were 2.80% and 1.68% (n = 6). The contents of the two fructo- oligosaccharides in chicory root, artichoke and oral liquid were also determined. The results shows chicory root possess the highest GF2 and GF3 contents in all vegetable tissues, 6.61g/100g dw and 6.51g/100g dw, whereas the highest composition of kestose and nystose in food was found in Quantum FOS powder (raw material for additives), 34.13g/100g and 32.12g/100g respectively. The method recoveries of GF2 and GF3 were 93.97% and 97.14%. This method was sensitive, selective, and simple for FOS quantification in vegetables and food products.